The PAM dependent recruitment of the Cas9-sgRNA complex triggers the initiation of the DNA-sgRNA heteroduplex
R-loop formation .
For example, MTHFR c.665C>T genotyping at 0.13[degrees]C/s had only a small heteroduplex
Despite the negative report for the third isolation, no heteroduplex
production and single base Displacement were seen on PAGE even after recheck and doubling the incubation time in the EDTA buffer (Fig.
Fifteen novel FBN1 mutations causing Marfan syndrome detected by heteroduplex
analysis of genomic amplicons.
Heterozygous DNA samples formed heteroduplexes
, resulting in a different shape of the melting curve compared with a homozygous sample.
The first lowest band was 191 bp DNA of normal alleles, the middle band represented 250 bp mutant DNA of exon-11, and the upper band was 300 bp heteroduplex
DNA arising from the combination between normal and mutant DNA alleles, as shown in Figure 4.
Imberti, "Assessment of T-cell receptor [beta]-chain diversity by heteroduplex
analysis," Human Immunology, vol.
This approach requires a treatment of the amplified DNA with CEL1 endonuclease or any of a number of single strand endonucleases, after heteroduplex
formation between the DNA of lines to be investigated.
Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex
However, errors can occur due to allelic dropout, where females are miss-sexed as males (Arnold et al., 2003; Robertson and Gemmell, 2006) or due to the formation of heteroduplex
DNA molecules, where males can be misidentified as females (Casey et al., 2009).
We analysed the BRCA2 gene using a novel high-throughput multiplex fluorescence heteroduplex
detection system developed for the ABI3130xl genetic analyzer.