Determination of hexokinase
isoenzyme I and II composition by RT-PCR: increased hexokinase
isoenzyme II in human renal cell carcinoma.
, glucose-6phosphatase and phosphorylase levels in hereditarily obesehyperglycemic mice.
NO.) ABBREVIATION Adenylate kinase (22.214.171.124) ADK Aspartate aminotransferase (126.96.36.199) AAT-1 AAT-2 Catalase (188.8.131.52) CAT Glucose-6-phosphate Dehydrogenase (184.108.40.206) G6PDH Glucose-6-phosphate Isomerase (220.127.116.11) GPI Glutamate Dehydrogenase (18.104.22.168) GTDH Hexokinase
(22.214.171.124) HK Isocitrate Dehydrogenase (126.96.36.199) IDH Malate Dehydrogenase (188.8.131.52) MDH-1 MDH-2 Malate Dehydrogenase (NADP+) (184.108.40.206) MDHP-1 MDHP-2 Mannose Phosphate Isomerase (220.127.116.11) MPI Phosphoglucomutase (18.104.22.168) PGM Table 2 Allele frequencies in specimens of Euglandina texasiana (San Fernando and Mission), E.
(14) In the GLUT pathway, benzaldehyde is met immediately by the enzyme hexokinase
Il (HK2) and, through enzymatic reaction with ATP, is changed to glucose 6-phosphate-benzaldehyde (G 6-p-b).
All samples were analyzed using following methods: glucose, triglycerides and total cholesterol were analyzed using hexokinase
, GPO-PAP and CHOD-PAP methodologies respectively.
Fasting blood sugar (FBS) by Hexokinase
method and free iron by direct iron assay using chromophore ferene[R] were analyzed in blood sample using standard kits in Dimension RxL autoanalyzer, USA and HbAlc by high-performance liquid chromatography method was analyzed in blood sample using Bio-Rad autoanalyzer, USA.
2-DG has been extensively described to competitively inhibit hexokinase
(HK) and glucose phosphoisomerase (PGI) in solid tumors .
Antibodies against complex I~V, hexokinase
2 (HK2), phosphofructokinase (PFKM), pyruvate kinase 2 (PKM2), and [beta]-actin were obtained from Proteintech Group (Chicago, IL, USA).
The targets of HIF-1 include hexokinase
II , angiogenic growth factors (e.g., VEGF), haematopoietic factors (e.g., erythropoietin and transferrin) , and membrane transporters including glucose transporter-1 (GLUT-1) and monocarboxylate transporter-4 (MCT-4).
Proteins identified by mass spectrometry were marked: CPS--carbamoyl phosphate 1, HXKI-- hexokinase
I, GRP78--78 kDa glucose-regulated protein precursor, Lam--lamin A/C, Mor--mortalin, H2B--histone 2B, H4--histone.
Glucose analysis was performed in a central laboratory using a hexokinase
method on a Siemens Advia 1800.
Blood glucose was estimated by hexokinase
method on Olympus AU2700 analyzer.