Histochemistry

(redirected from histochemical)
Also found in: Dictionary, Medical.

histochemistry

[¦hi·stō′kem·ə·strē]
(biochemistry)
A science that deals with the distribution and activities of chemical components in tissues.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Histochemistry

 

the division of histology that studies the chemical properties of animal and plant tissue.

Histochemistry seeks to elucidate the characteristics of metabolism in tissue cells and interstitial media. It studies changes in cell properties in the process of development and the connection between the work, metabolism, and renewal of mature cells and tissues. A basic principle of histochemical methodology is the bonding of a definite chemical component of cells with a stain or the formation of a stain in the process of the reaction. A number of methods (cytophotometry and luminescence and interference microscopy) are based on the physical properties of substances. Various histochemical methods determine the localization and quantity of many substances in tissue and their metabolism (tissue autoradiography), bonds with submicroscopic structure (electron histochemistry), and enzyme activity. Immunohistochemistry is a promising trend. The most precise histochemical methods, which permit investigation of cell structure, are called cytochemical methods.

The first special histochemical research was done by the French scientist F. Raspail (1825-34). Histochemistry began to develop intensively beginning in the 1940’s, when reliable methods appeared for determining proteins, nucleic acids, lipides, polysaccharides, and certain inorganic components in the cell. By means of histochemical methodology it has been possible, for instance, to show for the first time the connection of changes in the quantity of RNA with the synthesis of protein and the constancy of DNA content in the chromosome bank.

REFERENCES

Pearse, E. Gistokhimiia: Teoreticheskaia i prikladnaia. Moscow, 1962. (Translated from English.)
Burstone, M. Gistokhimiia fermentov. Moscow, 1965. (Translated from English.)

V. IA. BRODSKII

The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
Evaluation of a direct, rapid immune histochemical test for rabies diagnosis.
Histochemical tests indicated that the cuticle does not rupture during secretion.
Until today, it is well known in many published articles in different birds that the carbohydrates of gastric mucus in the proventriculus and gizzard histochemical are different from each other (Aitken, 1958; Pastor et al., 1988; Selvan et al., 2008; Kadhim et al., 2011; Prasad and Kakade,1990; Hassan and Moussa, 2012).
Histochemical differentiation of microfilariae of Dirofilaria immitis, Dirofilaria repens, Dipetalonema sp.
Dhale, "Histochemical investigation on some medical plants," Advance Research in Pharmaceuticals and Biological, vol.
The histochemical tests carried out in the gut of Centropomus revealed the presence of neutral and acid glycoproteins produced by epithelial cells that may protect the mucosa of the acid contained in the stomach.
Structural and histochemical aspects: The GTs peduncle cells are short and cylindrical (Fig.
In 2006, we began a quest to locate and develop a simple, effective, and reproducible procedure which would decrease the variables in the histochemical procedure, using flow cytometry (FCM).
ABSTRACT We applied quantitative histochemical techniques and digital image analysis to study seasonal cycles of use of lipid and protein reserves during vitellogenesis in the pearl oyster Pinctada mazatlanica.
At the same time, lectins are histochemical probes used to identify the specific terminal ends of the carbohydrate sequences of oligosaccharides (Schulte & Spicer, 1983).