Quantitatively, the number of possible hybrids will depend on the number of original homoduplex
molecules present in the mixture.
and hetroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus Group".
HLA-DQA1 and -DQB1 genotyping by PCR-RFLP, heteroduplex and homoduplex
If a mutation exists, the melting temperature of the heteroduplex will be lower than that of the homoduplex
Detection of clarithromycin-resistant Helicobacter pylori strains by a preferential homoduplex
Conformation-sensitive capillary electrophoresis (CSCE)  is a simple method for indirect mutation scanning that is based on the principle that homoduplex
and heteroduplex DNA have different electrophoretic mobilities under partially denaturing conditions (19-21).
This method takes advantage of the dramatic drop in PCR amplification that occurs for perfectly matched homoduplex
templates when the denaturation temperature is less than the critical denaturation temperature ([T.
As mentioned above, it is possible that high-abundance mutants may become less differentiated from the wild type if enrichment is quite strong and if their homoduplex
melting curves are comparable to the wild-type melting curve (4).
The altered melting curve shape is a result of the presence of both heteroduplex and homoduplex
amplicons in the PCR product, vs only homoduplexes
from normal samples.
If no SNP is present, all of the homoduplex
DNA fragments elute as a single peak on the chromatogram.
Different homozygotes each produce only a single homoduplex
For dHPLC, it has been shown (1) that temperature conditions can be optimized by use of predictive software to improve the separation of heteroduplex and homoduplex
peaks and achieve high-resolution results.