Assessment of Catalase (CAT): Tissue homogenate
(0.1 ml) was mixed with phosphate buffer (1 ml) and hydrogen peroxide (0.4 ml) and later on mixed with dichromate acetic acid reagent (2 ml).
In the brain homogenates
, the levels showed an increase in the RC (P<0.001) and DR (P<0.001) groups.
extraction was carried out on JHBE-50A homogenate
extractor (Golden Star Technology, Inc., Ltd., Zhengzhou, China).
As noted, a significant increase in the amount of this protein was detected after 3 and 4 hours of perfusion in homogenates
of these hearts.
In terms of spatial learning performance in Morris water maze test, matching the results from assays of sialic acid levels, the cubs whose female parental generation individuals were orally administered high-dose EBN homogenate
during pregnancy or both pregnancy and lactation periods exhibited better memory function and level of intelligence.
was centrifuged at 5000 rpm for 5 min.
Then the homogenates
were centrifuged at 4[degrees]C for 20 min at 15,000xg, the supernatant was collected for sarcoplasmic protein analysis.
Property PMCA RT-QuIC Prion disease vCJD Sporadic, familial, specificity and acquired [PrP.sup.C] Transgenic mouse Truncated recombinant substrate brain homogenate
hamster [PrP.sup.C] expressing human (2nd generation assays) [PrP.sup.C] Fragmentation Sonication Shaking technique Fluids and tissues Blood, urine CSF, nasal brushings tested Detection Western blot Thioflavin T fluorescence Turnaround time [congruent to]11 days <24 h Currently in No Yes clinical use
Application of the method to rat brain tissue homogenate
To investigate the potential relative therapeutic effects of Crataegus aronia and simvastatin on upregulation the antioxidant GSH and suppression the oxidative stress TBARS and lipidemia, we measured reduced GSH and TBARS biomarkers in liver homogenates
and lipid fractions (cholesterol, triaceylglycerol, and low density lipoprotein (LDL)) in the blood of rats sacrificed after 15 weeks (Table I).
To determine the relative sensitivity of PMCA, we retitrated a reference sample (10% cerebral cortex homogenate
from a vCJD-affected patient) that had previously undergone endpoint titration (IC inoculation route; Table 1) in bovine PrP-expressing mice (tgBov).
 After analyzing the test samples, the remaining homogenates
were mixed and then equal volume of homogenate
mixture was added to different standard concentrations.