Homogenate


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homogenate

[hə′mäj·ə·nət]
(biology)
A tissue that has been finely divided and mixed.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Homogenate

 

a suspension of subcellular particles of pulverized animal or plant tissue in a buffer or other solution; the suspension is usually obtained with the aid of a special device called a homogenizer. Differential centrifugation, among other methods, is used to separate such microscopic cell structures as organelles and such molecular structures as proteins, nucleic acids, and lipids from the homogenate. Homogenates are also used to study biochemical reactions outside an organism (in vitro).

The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
Assessment of Catalase (CAT): Tissue homogenate (0.1 ml) was mixed with phosphate buffer (1 ml) and hydrogen peroxide (0.4 ml) and later on mixed with dichromate acetic acid reagent (2 ml).
In the brain homogenates, the levels showed an increase in the RC (P<0.001) and DR (P<0.001) groups.
Homogenate extraction was carried out on JHBE-50A homogenate extractor (Golden Star Technology, Inc., Ltd., Zhengzhou, China).
As noted, a significant increase in the amount of this protein was detected after 3 and 4 hours of perfusion in homogenates of these hearts.
In terms of spatial learning performance in Morris water maze test, matching the results from assays of sialic acid levels, the cubs whose female parental generation individuals were orally administered high-dose EBN homogenate during pregnancy or both pregnancy and lactation periods exhibited better memory function and level of intelligence.
Then the homogenates were centrifuged at 4[degrees]C for 20 min at 15,000xg, the supernatant was collected for sarcoplasmic protein analysis.
Property PMCA RT-QuIC Prion disease vCJD Sporadic, familial, specificity and acquired [PrP.sup.C] Transgenic mouse Truncated recombinant substrate brain homogenate hamster [PrP.sup.C] expressing human (2nd generation assays) [PrP.sup.C] Fragmentation Sonication Shaking technique Fluids and tissues Blood, urine CSF, nasal brushings tested Detection Western blot Thioflavin T fluorescence Turnaround time [congruent to]11 days <24 h Currently in No Yes clinical use
To investigate the potential relative therapeutic effects of Crataegus aronia and simvastatin on upregulation the antioxidant GSH and suppression the oxidative stress TBARS and lipidemia, we measured reduced GSH and TBARS biomarkers in liver homogenates and lipid fractions (cholesterol, triaceylglycerol, and low density lipoprotein (LDL)) in the blood of rats sacrificed after 15 weeks (Table I).
To determine the relative sensitivity of PMCA, we retitrated a reference sample (10% cerebral cortex homogenate from a vCJD-affected patient) that had previously undergone endpoint titration (IC inoculation route; Table 1) in bovine PrP-expressing mice (tgBov).
[18] After analyzing the test samples, the remaining homogenates were mixed and then equal volume of homogenate mixture was added to different standard concentrations.