Homogenate

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homogenate

[hə′mäj·ə·nət]
(biology)
A tissue that has been finely divided and mixed.

Homogenate

 

a suspension of subcellular particles of pulverized animal or plant tissue in a buffer or other solution; the suspension is usually obtained with the aid of a special device called a homogenizer. Differential centrifugation, among other methods, is used to separate such microscopic cell structures as organelles and such molecular structures as proteins, nucleic acids, and lipids from the homogenate. Homogenates are also used to study biochemical reactions outside an organism (in vitro).

References in periodicals archive ?
However, these bacteria were cultured from whole homogenates of bed bugs, and standard vector competence trials were not conducted.
We developed an indirect ELISA to quantitate relative amounts of IBA-1 expression in brain homogenate [for details, see Supplemental Material, p.
The initial tier of assays demonstrated that crude insect homogenates were highly virulent to second through fourth instars.
The activity of acetyl-CoA carboxylase in mammary gland nuclei-free homogenates prepared in the presence and absence of avidin indicates that polymerization/ depolymerization are not solely responsible for the modification of activity (Table 4).
T] are the volumes of saline and tissue in the homogenate, respectively.
More recently, differential mRNA expression of cytokeratin 19 (CK19) and mammaglobin in breast epithelial cells versus lymphoid cells has been used to develop a real-time RT-PCR assay (GeneSearch[TM] BLN Assay, Veridex, LLC, Warren, NJ) to detect sentinel lymph node metastasis intraoperatively by assessing the homogenate of the nodal tissue [27-31].
On the other hand, no cytotoxic activity was seen in the spleen homogenates obtained from CrP fed mice in comparison to normal control mice.
Lipid peroxidation was analyzed by measuring thiobarbituric acid-reactive substances (TBARS) in homogenates, as previously described by Draper and Hadley (1990).
The most commonly used technique for measuring complex I is a spectrophotometric assay measuring rotenone-sensitive NADH oxidation at 340 nm in tissue homogenate or mitochondria-enriched fractions from cultured fibroblasts (3, 4); however, the sensitivity and specificity of these assays are not optimal.
25 was utilized in the next set of experiments, and appropriate amounts of labeled calpain and Mab were added to the meat homogenates, which were then spiked with calpastatin.
The homogenates were centrifuged at 14,000g for 2 min, and the protein concentrations of the supernatant and pellet fractions were determined using a dye binding method (Bradford, 1976).