Preparation of Tissue Homogenates
. After being acclimatized for one week, the animals were sacrificed by decapitation, and their livers, kidneys, brains, and hearts were collected and weighed and put in phosphate buffer (0.1 mol/L, pH 7.8) supplemented with KCl (1.5%) in an ice bath.
In this regard, we observed in this study a decrease in the expression of NKCC2 both in cortex and in medulla homogenates
at day 2 after release of BUO, which might precede the dramatic decrease at day 3 after release of BUO described by Li et al.
The tumor formation rate was also 100% (4/4) in the nude mice inoculated with the tumor tissue homogenate
. No subcutaneous swelling or tumors were observed in the nude mice inoculated with the less concentrated MKN-45 cell suspension (200 [micro]L, 1 x [10.sup.7] cells/mL).
The concentration of the GSH and some oxidative stress-related factors were also detected by ELISA in the hepatocyte homogenates
. The GSH production in the hepatocytes was inhibited in the pSS group (p = 0.0034, Figure 3B) and elevated by the GSH injection (p = 0.0264).
75 [micro]g adipocyte homogenate
was applied directly to a nondenaturing 10% polyacrylamide gel.
Additionally, tissue homogenates
weighing 0.1 g were taken from a separate region of each sample of foot muscle, hypobranchial, rectal, prostate, and capsule glands.
CSE, TLR4, IL-10, IL-12, and TNF-[alpha] levels in the renal homogenates
were determined using a rat enzyme-linked immunoadsorbent assay (ELISA) kit and commercially available antibodies (R&D Systems, USA).
Kidney, testis, spleen and jejunum were weighed and homogenized immediately to give 50% (w/v) homogenate
in ice- cold medium containing 50 mM TrisHCl and 300 mM sucrose, pH 7.4 (Tsakiris et al., 2004).
ESL and injection of eyestalk homogenates
did not affect hemolymph osmolality in C.
To 200 [micro]l of the tissue homogenate
, 500 pi of 0.8% thiobarbituric acid in water (w/v) and 1.25 ml of 20% trichloracetic acid (w/v) were added, and the mixture was incubated for 1 hour in a water bath.
For tissue homogenate
, each weighed tissue sample was thawed and the homogenized in ice-cold physiological saline (2 mL).