However, these bacteria were cultured from whole homogenates
of bed bugs, and standard vector competence trials were not conducted.
GSH level in the liver homogenate
was determined by the method of Moron et al.
We developed an indirect ELISA to quantitate relative amounts of IBA-1 expression in brain homogenate
[for details, see Supplemental Material, p.
The initial tier of assays demonstrated that crude insect homogenates
were highly virulent to second through fourth instars.
The activity of acetyl-CoA carboxylase in mammary gland nuclei-free homogenates
prepared in the presence and absence of avidin indicates that polymerization/ depolymerization are not solely responsible for the modification of activity (Table 4).
T] are the volumes of saline and tissue in the homogenate
More recently, differential mRNA expression of cytokeratin 19 (CK19) and mammaglobin in breast epithelial cells versus lymphoid cells has been used to develop a real-time RT-PCR assay (GeneSearch[TM] BLN Assay, Veridex, LLC, Warren, NJ) to detect sentinel lymph node metastasis intraoperatively by assessing the homogenate
of the nodal tissue [27-31].
On the other hand, no cytotoxic activity was seen in the spleen homogenates
obtained from CrP fed mice in comparison to normal control mice.
Lipid peroxidation was analyzed by measuring thiobarbituric acid-reactive substances (TBARS) in homogenates
, as previously described by Draper and Hadley (1990).
The most commonly used technique for measuring complex I is a spectrophotometric assay measuring rotenone-sensitive NADH oxidation at 340 nm in tissue homogenate
or mitochondria-enriched fractions from cultured fibroblasts (3, 4); however, the sensitivity and specificity of these assays are not optimal.
25 was utilized in the next set of experiments, and appropriate amounts of labeled calpain and Mab were added to the meat homogenates
, which were then spiked with calpastatin.
were centrifuged at 14,000g for 2 min, and the protein concentrations of the supernatant and pellet fractions were determined using a dye binding method (Bradford, 1976).