The horse serum
and rabbit serum have been produced for the first time in our country," Managing-Director of a knowledge-based company for medical products Amir Khani told FNA on Tuesday.
All four clinical samples received on ice were plated on Blood agar (a) (containing 5% Sheep RBCs), Brucella selective agar (a) [containing 5% v/v inactivated horse serum
(RM1239) and Brucella selective supplement (FD005)] and on MaConkey agar (a) and incubated anaerobically in an atmosphere of 5-10% C[O.sub.2] for seven days.
To determine the infection prevalence of EqPV-H, we tested 100 horse serum
samples of convenience submitted to the New York State Animal Health Diagnostic Center at Cornell University for nonclinical reasons.
Myoblasts were differentiated by shifting medium to DMEM containing 2% horse serum
. Cells were maintained in differentiation media for 96 h.
SCs were gradually differentiated from parallel arrangement of myocytes after 24 h to 96 h induction of 2% horse serum
(Figure 6A to 6D).
For the lowest concentration pool, the human serum matrix was further diluted with horse serum
to reach the anticipated concentration, and 2 controls (approximately 10 and approximately 100 ng/L, respectively; PreciControl Varia) were used in randomized order of all samples on the system.
The active culture (10ml) of the organism (48 hours at 37degC incubated culture) was inoculated in a flask of 2.5 liter capacity containing 100ml sterile Frey's broth with 10 percent horse serum
At 90% confluence, the media were changed to DMEM plus 2% horse serum
(HyClone Company, USA) to induce myotube formation.
These include monoclonal antibodies, RNA-polymerase inhibitors, small interfering RNAs targeting RNA-polymerase, inhibitors of viral mRNA translation, or hyper-immune horse serum
and bovine fetal serum were obtained from Gibco Life Technologies (USA).
Commonly used additives in the maintenance cell culture medium are fetal calf serum (FCS), goat serum, horse serum
, allontoic fluid, colostral whey, oval albumin, etc.