housekeeping gene


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housekeeping gene

[′hau̇s‚kēp·iŋ ‚jēn]
(genetics)
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According to the evaluation, all housekeeping gene transcriptions in the midgut were normalized to the transcription of EF1[alpha].
2003) Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR.
To allow a comparison between individual samples, we calculated the ratios of housekeeping gene RNAs.
Discrepancies between our results and the results of previous studies can be attributed to the use of different methods for the quantification of VEGF and, in cases when RT-PCR is used, to the housekeeping gene selected for the normalization of results.
We report here an RT-PCR-based assay combining the determination of expression of both human IMPDH isoenzymes based on normalization to a housekeeping gene index.
The presence of nine housekeeping genes per array allowed us to discard housekeeping genes that were induced or repressed by a particular treatment (typically one to two of nine included on the array).
beta]-Actin, a housekeeping gene commonly used to normalize gene expression assays, was also downregulated on our arrays for fish treated with [E.
Primers for swine FTO (GenBank: NM_001112692) and housekeeping gene (GAPDH) were designed by Oligo 6 software and synthesized by Introvigen company (Shanghai, China).
9%) considered to be of poor quality because of low housekeeping gene concentrations (see the section on the housekeeping gene below), tissue specimens from 155 breast cancer patients remained for KLK7 expression analysis.
The resulting IOD values were used to generate a relative IOD that is the ratio of the respective heat shock protein reaction product to that of the housekeeping gene g3pdh.
This index was then compared to each individual candidate housekeeping gene by pair-wise correlation analyses, with each combination assigned a value for the Pearson correlation coefficient (r) and the probability (p) (Pfaffl et al.