housekeeping gene


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housekeeping gene

[′hau̇s‚kēp·iŋ ‚jēn]
(genetics)
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Validation of Housekeeping Genes. All tested reference genes possessed similar PCR efficiencies (Table 1).
When testing effects of RSV on the expression pattern of exons of the BDNF gene in the rat embryonic hippocampi, we were encountered with dramatic changes in the expression of housekeeping genes. Although, we tried three different housekeeping genes reported to be the most stable ones, (36) but all of them showed instability in our RSV administered groups.
Data are expressed as the mean of [DELTA]Ct [+ or -] SEM relative to the 20 h time point and normalised against the housekeeping gene GAPDH.
The expression data for each gene was divided by that for the YWHAZ housekeeping gene. The median number of total mutations per Mb (left panels) or the median number of SNP-type mutations per Mb (right panels) was analyzed, and the Spearman rank correlation coefficient ([rho]) and P values were provided.
Table 1: Housekeeping genes used as internal control for real time PCR analysis
The sequences for each housekeeping gene were aligned by the MUSCLE algorithm and concatenated in MEGA 5.1 [17].
Specific primers for determining the expression of housekeeping genes and the genes of interest were designed using Primer 3 software.
pneumoniae strains to compare them and confirm clonal and pathogenicity relationships through housekeeping gene analysis.
Relative quantification was performed using an housekeeping gene as reference.
"Now we know that specific amino acid residues in this loop are responsible for making this from a normal housekeeping gene into something that's capable of degrading this class of antibiotics," Nair stated.