The first involved grafts between soybean and mung bean, and the second involved grafts between soybean and hyacinth bean. Seeds were surface sterilized by immersing in 950 mL [L.sup.-1] ethanol for 10 s, followed by 4 min treatment with 10 g [kg.sup.-1] sodium hypochlorite, and then rinsed four times with sterilized, purified (reverse osmosis) water.
Reciprocal grafts involving mung bean shoots and soybean roots, and hyacinth bean shoots and soybean roots, were also made but not used in the current experiment.
Given that the seedling size of hyacinth bean was approximately twice that of mung bean at the time of grafting (reflecting larger seed size of hyacinth bean), the similar shoot mass of self-grafted hyacinth bean and mung bean plants at harvest (Table 1) indicates that mung bean self grafts were more compatible.
For hyacinth bean grafted combinations, similar nodule numbers occurred for self-grafted hyacinth bean and for Williams 82 soybean shoots grafted to hyacinth bean roots.
With the hyacinth bean graft combinations, the USDA 4787 strain tended to induce greater nodule mass, but was significantly greater in only the hyacinth bean self graft.
Nodulation response of self-and interspecie-graft combinations of mung bean, soybean, and hyacinth bean. Values shown are means [+ or -] SD.
The NOD1-3-hyacinth bean graft also showed greater partitioning to nodules than in other graft combinations involving hyacinth bean roots.
Has: cardinal climber (hummingbird vine), four o'clocks (bicolor), hyacinth beans
, cayenne pepper, prickly pear and flowering cabbage.