35) Moreover, the characterization a transferable hybrid plasmid pAPEC-O103-ColBM encoding multidrug resistance and pathogenicity was recently described.
Sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal Escherichia coli.
African Patient age, y patient name CNM4839/03 ([dagger]) No 3 H051860415 No 38 H070360201 ([double dagger]) Yes 35 H070420137 ([double dagger]) Yes 35 H073180204 Yes 34 H091340084 No 59 H091800482 Yes 30 H095100307 ([section]) Yes 68 H100240198 ([section]) Yes 68 FI101700366 ([section]) Yes 68 * All isolates contained a virulence-resistance hybrid plasmid similar to pUO-SeVR1.
In recent years, in association with multidrug resistance, another trend has arisen: the emergence of virulence-resistance (VR) plasmids; these are hybrid plasmids that harbor resistance (R) and virulence (V) determinants.
This DNA is also prepared from another pBR322-SV40 hybrid plasmid
containing a PstI site in the SV40 region by PstI restriction endonuclease digestion, oligo(dG) tailing with TdT, HindIII digestion and purification by agarose gel electrophoresis.