Antibodies were depleted from the hybridoma
supernatant by the indicated procedures.
Anti-Plexin C1 monoclonal antibodies were purified from hybridoma
supernatants on AKTA-Purifier Chromatography (GE, Massachusetts, USA) using protein G affinity column (HiTrap protein G, GE).
In addition, all hybridoma
supernatants were tested against parainfluenza virus (negative control) respiratory tract cell antigens at the same concentration, with overnight incubation at 4 [degrees]C.
Keywords: Domoic acid, Amnesic shellfish poisoning, Cell culture, Hybridoma
, Monoclonal antibody, Fetal bovine medium, Carbohydrates.
and Jessell, T, respectively, were obtained from the Developmental Studies Hybridoma
Bank, created by the NICHD of the NIH and maintained at Department of Biology, The University of Iowa, Iowa City, IA 52242.
Pursch, "Human x (mouse x human) hybridomas
stably producing human antibodies," Hybridoma
supernatant was added to the plates, and incubated for 12 h at 4[degrees]C.
The effect of hybridoma
concentration on the yield of antibody-producing clones.
The living substrate may be genetically modified in a number of ways to provide the required active ingredient, including recombinant DNA technology or hybridoma
The study of activity of AIAT produced by them with the help of ELISA test revealed that AIAT produced by the 2D9, 2G9 hybridoma
cells preserved their activity.
(1,7,11-18) We herein describe the development of a new mouse monoclonal SOX10 hybridoma
(BC34) and evaluate its sensitivity and specificity in normal and neoplastic tissues, with an emphasis on melanoma and its subtypes.
Experimental support was provided by the Hybridoma
Facility of the Rheumatic Diseases Core Center.