The nucleophilic attack of the base on the electrophilic carbon atom of the hydrazide
molecule results in the formation of a hydrazine moiety.
The benzoic acid hydrazide
1 (1.35 g, 10.02 mmol) dissolved in 25 ml of absolute ethanol was added to the above solution in portionwise with constant stirring.
* Kucukguzel G, Kocatepe A, De Clercq E, Sahin F, Gulluce M (2006) Synthesis and biological activity of 4-thiazolidinones, thiosemicarbazides derived from diflunisal hydrazide
. Eur J Med Chem 41: 353-359.
Synthesis of the carbohydrazide derivatives To a solution of hydrazide
(1 mmol) (2) and triethylamine (2 mmol) in dry C[H.sub.2][Cl.sub.2] (5 mL), a solution of previously prepared benzoyl chloride (1 mmol) was added dropwise at room temperature.
Phenylalanine acid hydrazide
(3b): Rf=0.69 (CH[Cl.sub.3]/MeOH 4/1); yield 92%; thick syrup; IR (KBr v max[cm.sup.-1]): 3303 (N-H), 3055 (=C-H), 2927(C-H), 1618 (C=O), 1510 (C=C), 1093 (C-N).
A mixture of hydrazide
3 (2.11g, 10 mmole) and formic acid (15 ml) was heated for 15 h (TLC) under reflux.
The synthetic scheme for the synthesis of the hydrazide
and its Schiff bases is shown in Figure 1.
Oxadiazole 4 was obtained by the reaction of acid hydrazide
(Almasirad et al., 2011) with carbon disulphide in alkaline medium under reflux conditions.
The conventional method for amino and hydrazide
group quantification includes the 2,4,6-trinitrobenzenesulfonic acid (TNBS) test, which comprises three steps involving work with toxic reagents such as TNBS and methanol.
Kim, "A relative and absolute quantification of neutral N-linked oligosaccharides using modification with carboxymethyl trimethylammonium hydrazide
and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry," Analytical Biochemistry, vol.
The latter reaction gave three products, none of them being the expected hydrazide
The core reaction is achieved by refluxing equimolar quantities of Diclofenac hydrazide
III with Isatin nucleus a1 for 6 hours in Schiff's condensation reaction carried out in the presence of catalytic amount of glacial acetic acid.