Thus, ATP was degraded into xanthine or hypoxanthine
via IMP or inosine generation, and GTP was degraded into GMP and guanosine in parallel during the aging process, resulting in increased contents of uric acid and PRPP.
Maximum values of hypoxanthine
did not exceed 1.06 [micro]mol [g.sup.-1] in the 336th hour of storage, for the intensity in 150 volts.
iodinea cerata and body epithelium were analyzed by TLC, in parallel with pure guanine and hypoxanthine
Dipotassium hydrogen orthophosphate, potassium dihydrogen phosphate, creatinine, uric acid, hypoxanthine
, and sodium hydroxide were purchased from Riedel-de Haen AG, Seelze, Hannover.
, aminopterin, and thymidine (HAT) were used to culture NG108-15 cells; however, aminopterin in HAT was toxic to INS-1 cells (Figure 2(a)).
The enzyme xanthine oxidase catalyzes the oxidation of hypoxanthine
and xanthine to uric acid, producing a superoxide radical and hydrogen peroxide.
Analysis of correlation matrix showed high correlation of BCAAs and cysteine, PPP metabolites (except ribose 5-phosphate), and inosine and its metabolites (hypoxanthine
It is a competitive inhibitor of xanthine oxidase, which is an enzyme used to break down hypoxanthine
to xanthine and xanthine to UA.
In vitro analysis is comprised of microscopy-based assay (also known as WHO microtest), tritium-labeled hypoxanthine
assay (isotopic assays), fluorimetric assay (using DNA-specific dyes), cytometry-based assay, and enzyme-linked immunosorbent assay (ELISA) [31, 32].
The levels of cholesterol, myo-inositol-1-phosphate, uracil, and hypoxanthine
were higher in both serum and tissues of the EC patients than those of the controls.
Among other metabolites detected in AH (Table 6) several molecules with antioxidative properties (e.g., citric acid, ascorbic acid, quinic acid, pantothenic acid, betaine, or taurine) or related to oxidative stress (e.g., pyroglutamic acid or hypoxanthine
) were identified.
IMP was lower and ATP and hypoxanthine