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Related to immunoassays: ELISA, Enzyme immunoassay


An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay


A laboratory detection method that uses antibodies to react with specific substances.
References in periodicals archive ?
The report analyzes the European enzyme immunoassay (EIA) Market by type, technique, end-user, and by country.
Over 7 months, we analyzed a total of 1681 urine samples across 5 lots of the same CLIA-waived, lateral-flow immunoassay (ABMC RapidTOX[R]), according to manufacturer instructions.
Some studies have shown that many immunoassays do not completely measure 25-0H Vitamin D2 and that many assays are unable to reliably measure samples containing 25-0H Vitamin D2.
The other, published April 20 in Analytical Chemistry, demonstrates the effect in immunoassays.
In addition, although almost all of the participants using immunoassays with a cutoff of 300 ng/mL detected the opiate hydromorphone at 1000 ng/mL, the many laboratories that used fluorescent immunoassay (FIA) and microparticle immunoassay (kinetic interaction of microparticles in solution) did not (UDS-A 2003) (Table 2).
Nelson shockingly show that these immunoassays are just glorified [T.
The authors indicated that technologic advances have improved the specificity of detecting SV40 antibodies and used an enzyme immunoassay based on viruslike particles (VLPs) to perform the analysis (1).
Enzyme immunoassays are used widely in both clinical and research fields because they are rapid, simple, accurate, and specific.
The 2013 Quantitative Immunoassay Dashboard was developed from responses to a 24-question survey completed by 553 scientists located in North America and Europe.
Increasing Use of Immunoassays in Food Sciences and Quality
Various metabolites, including M-I (13-O-demethyl), M-II (31-O-demethyl), M-III (15-O-demethyl), and M-V (15,31, di-O-demethyl) crossreact with immunoassays for tacrolimus (8).
CEDIA is known worldwide for immunoassays in drugs-of-abuse screening, therapeutic drug monitoring (TDM), and specifically immunosuppressant drug (ISD) monitoring.