immunodiffusion


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immunodiffusion

[¦im·yə·nō·də′fyü·zhən]
(immunology)
A serological procedure in which antigen and antibody solutions are permitted to diffuse toward each other through a gel matrix; interaction is manifested by a precipitin line for each system.
References in periodicals archive ?
[1.] Nonstandard abbreviations: ANA, antinuclear antibody; IIF, indirect immunofluorescence; ENA, extractable nuclear antigen; SLE, systemic lupus erythematosus; ID, immunodiffusion; LIA, line immunoassay; and CI, confidence interval.
(23) Radial Method Immunonephelometry immunodiffusion Gc2-2 226 [+ or -] 3 247 [+ or -] 3 [183-268] (a) (n = 11) (n = 40) (b) Gc2-1 249 [+ or -] 2 284-3 [201-310] (n = 41) (n = 228) Gc1-1 272 [+ or -] 2 305 [+ or -] 3 [218-346] (n = 48) (n = 316) Mean [+ or -] SE, mg/L Daiger et al.
Electroimmunoassay, radioimmunoassay, and radial immunodiffusion assay evaluated for quantification of human apolipoprotein B.
Demonstration of type-specific influenza antibody in mammalian and avian sera by immunodiffusion. Bull World Health Organ.
Radial immunodiffusion disks were prepared by dissolving 30 mL/L of a specific antiserum against human immunoglobulins (IgG, IgM, and IgA) and [[alpha].sub.2]-MG in 2% agarose.
Among serologic tests, immunodiffusion and complement fixation for anti-Histoplasma antibody detection are widely applied; sensitivity rates are 95%-100% and 82%-90% for pulmonary and disseminated histoplasmosis, respectively.
Although CIE together with double immunodiffusion has represented the most widespread method for years, its further use seems unlikely mostly because of its poor sensitivity for some autoantibodies; on the other hand, the dot-blot method offers ease of performance as well as direct interpretation, and produced excellent results, features that predict its much wider use in clinical laboratories.
The double immunodiffusion technique was used to measure tube precipitin-type and complement fixing--type anti-coccidioidal antibodies (12,13).
Commonly used quantitative methods include microchromatographic techniques and automated HPLC for Hb [A.sub.2] (3,4), and alkaline denaturation, radial immunodiffusion, and automated HPLC for Hb F (3).
Performance comparison of immunodiffusion, enzyme-linked immunosorbent assay, immunochromatography and hemagglutination for serodiagnosis of human pythiosis.