Serological tests: immunodiffusion
, complement fixation, and latex agglutination test
In the present case, studies for an infectious etiology were negative except the positive serum Coccidioides IgM/ IgG by EIA and immunodiffusion
Overall, among 24 patients without a history of travel, 11 received a diagnosis only on the basis of positive immunoglobulin M or qualitative enzyme immunoassay or immunodiffusion
tests, and four received a diagnosis on the basis of positive coccidioidomycosis cultures (all of the latter were immunocompetent).
Two common ancillary tests used in the confirmation of blastomycosis are Blastomyces antigen and Immunodiffusion
In double immunodiffusion
test, a white band was observed between the two wells of antigen and antibodies for both the PLE-treated and piperine-treated mice serum because of the antigen-antibody reactions (Fig.
Layers inoculated with the LPAIV A/Bobwhite Quail/Pennsylvania/20304/98 (H7N2) were tested for antibody levels in the serum and egg yolk by using the agar gel immunodiffusion
test (AGID), hemagglutination-inhibition test (HI), anda commercially available enzyme-linked immunosorbent assay (ELISA).
Specificity of the rabbit antibody was tested using a double immunodiffusion
test (Ouchterlony & Nilsson 1973) and ELISA.
Immunoglobulins were determined by radial immunodiffusion
by the method of Manchini (1965) using commercial antisera to immunoglobulins A, M, G and E (production of Research Institute name after Gamaleya, Russian Federation).
Serology for H capsulatum, obtained for the evaluation of fever of unknown origin, revealed mycelial and yeast complement fixation titers of 1:512 with a positive M and H band on immunodiffusion
42-44) Attempts to develop serological assays using native PGL-I were at first problematic due to the apolar nature of the purified glycolipid and its lack of solubility in aqueous buffers commonly used in immunodiffusion
or ELISA assays.
Agar Gel Immunodiffusion
(AGID) Test (after six months of age): Detects circulating antibodies in the blood.
Standardization and validation of an agar gel immunodiffusion
test for the diagnosis of equine infectious anemia using a recombinant p26 antigen.