immunoelectrophoresis


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Immunoelectrophoresis

A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by reaction with specific antibodies.

A medium such as agar is deposited on a convenient base, for example, a microscopic slide. A small well is cut in the medium. A test solution is deposited in the well, and the contained substances are separated by electrophoresis along one axis of the plate. A trough is then cut in the medium parallel to, but at some distance from, the line of the separated substances. The trough is filled with antiserum which contains antibodies to one or more of the separated substances. The antiserum and substances diffuse toward one another and, where they meet, form curvilinear patterns of precipitation. These can be seen directly in clear media or can be visualized after washing out unreacted materials and staining in opaque media. See Immunoassay

immunoelectrophoresis

[¦im·yə·nō·i‚lek·trə·fə′rē·səs]
(immunology)
A serological procedure in which the components of an antigen are separated by electrophoretic migration and then made visible by immunodiffusion of specific antibodies.
References in periodicals archive ?
Production of rabbit antiserum against Trichomonas vaginalis parasite was confirmed in immunoelectrophoresis (Figure 1).
Concentration Diameter of ring ([micro]g/ml) (mm) 50 0.5 75 1 100 7 125 12 150 17 Table 3: Rocket immunoelectrophoresis. Antigen concentration Rocket height (in mg/ml) (in mm) 50 1.6 75 3.9 100 5.3 125 7.4 Table 4: Reproducibility of allergen reactions by ELISA.
In the first attempt to identify BT, Arlian et al, (15) used crossed immunoelectrophoresis to identify IgE binding antigens.
These investigations included a complete blood cell count, measurement of serum calcium and creatinine levels, serum protein electrophoresis and immunoelectrophoresis, urinalysis for Bence Jones protein, evaluation of [[beta].sub.2]-microglobulin, a skeletal survey with plain films (skull, ribs, humeri, and femurs), abdominal ultrasonography, bone marrow aspiration, and biopsy.
Therefore, when evaluating a plasma-cell neoplasm, it is critical to rule out multiple myeloma, which is done with a normal radiographic skeletal survey, negative serum and urine immunoelectrophoresis, and negative bone marrow biopsy (<5% plasma cells).
* Assessment tools also include toxicology screening, erythrocyte sedimentation rate, hemoglobin electrophoresis, fibrinogen, immunoelectrophoresis and antiphospholipid antibodies.
Agglutination, fluorescent antibody, and cross immunoelectrophoresis tests have all been used for diagnosing candidiasis.
Fractions obtained by gel filtration were further investigated by paper electrophoresis, analytical ultracentrifugation, immunoelectrophoresis in agar gels, double diffusion in agar gels, and immunotitration.
A total of 202 archaeological samples were submitted for cross-over immunoelectrophoresis (CIEP), an immunological technique originally developed in medico-legal work (Culliford 1964; Gaensslen 1983; Newman & Julig 1989).
Downs used crossover immunoelectrophoresis, Lowenstein radioimmunoassay, and the third group a dipstick clinicians use to detect hemoglobin in urine.
Cross-reactivity between the major and hypoallergen components was observed in immunoblotting and crossed-immunoelectrophoresis and fused-rocket immunoelectrophoresis using rabbit antisera to the purified major and hypoallergens, suggesting that the hypoallergen carries a dominant epitope of the major allergen.

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