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A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by reaction with specific antibodies.

A medium such as agar is deposited on a convenient base, for example, a microscopic slide. A small well is cut in the medium. A test solution is deposited in the well, and the contained substances are separated by electrophoresis along one axis of the plate. A trough is then cut in the medium parallel to, but at some distance from, the line of the separated substances. The trough is filled with antiserum which contains antibodies to one or more of the separated substances. The antiserum and substances diffuse toward one another and, where they meet, form curvilinear patterns of precipitation. These can be seen directly in clear media or can be visualized after washing out unreacted materials and staining in opaque media. See Immunoassay


A serological procedure in which the components of an antigen are separated by electrophoretic migration and then made visible by immunodiffusion of specific antibodies.
References in periodicals archive ?
Cross-over immunoelectrophoresis is the immunological method used in this analysis (Newman & Julig 1989).
Counter Immunoelectrophoresis against "arc-5" was performed in three cases with positive result obtained in all three.
The serum free light chain assay has been proven to be more sensitive than Immunoelectrophoresis (IFE) when used in conjunction with serum protein electrophoresis (SPEP).
Immunoelectrophoresis has been largely replaced by immunofixation.
Echinococcosis in people is diagnosed by imaging techniques such as ultrasonography, magnetic resonance imaging (MRI), or computed tomography (CT) scanning supported by positive serologic tests (ELISA, indirect immunofluorescence, immunoelectrophoresis, and immunoblotting).
Rule out generalized plasmacytoma * Bone marrow biopsy * Bone marrow scintigraphy * Skeletal roentgenography * Serologic and immunologic examinations: immunoelectrophoresis and measurements of liver and kidney function and calcium, paraprotein, and Bence Jones protein levels in urine and serum
Analysis of the conversion of the C3 and factor B precipitation line on immunoelectrophoresis of the serum treated with both ABP-F and ABP-M strongly suggested that these particles activated the complement system through the alternative pathway (Figures 1, 4, and 5).
28,60) Other specialized methods of platelet evaluation, such as crossed immunoelectrophoresis, will be discussed in the following section with the individual disorders for which they are useful.
Serum concentrations of total AGP were measured by rocket immunoelectrophoresis using goat anti-human specific AGP (Atlantic Antibodies, Stillwater MN).
These findings were confirmed by crossed immunoelectrophoresis (CIE) (Figure 3).
Nevertheless, the diagnosis of anaphylaxis should be established by various immunologic immunofluorescence reactions and immunoelectrophoresis hemagglutination.

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