Immunofluorescence

(redirected from immunofluorescence microscopy)
Also found in: Dictionary, Thesaurus, Medical.
Related to immunofluorescence microscopy: Confocal microscopy

Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
In order to study the quantity and cellular localization of the [beta]-catenin protein, we used indirect immunofluorescence microscopy.
The distribution of heavy chain deposits by immunofluorescence microscopy is similar to LCDD and displays a uniform, continuous pattern of linear or finely granular deposits in the renal ECM compartments (Figures 10 and 11).
Slow-growing fungi might be missed unless special detection techniques are used, such as immunofluorescence microscopy.
Both conventional lichen planus and its subtropicus variant have cytoid bodies that stain positive to IgG and IgM on direct immunofluorescence microscopy, Dr.
Confocal immunofluorescence microscopy of microtubules, microtubule-associated proteins, and microtubule-organizing centers during amphibian oogenesis and early development.
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy
Immunofluorescence microscopy revealed an absence of deposits of immunoglobulin, complements, and fibrinogen.
Tender are invited for Tests for immunofluorescence microscopy over a period of one year (in the specification)
Furthermore, immunofluorescence microscopy detected prominent mesangial IgA deposits with negative [C.
Benefit of adding antigen-specific assays to immunofluorescence microscopy.
Binding of the projectin-GFP fusion on pupal and adult muscle is detected using immunofluorescence microscopy.