Immunofluorescence

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Related to immunofluorescence microscopy: Confocal microscopy

Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
Immunofluorescence microscopy is usually negative, or at times, there may be focal, nonspecific positivity with immunoglobulins and complements.
Otomycosis: The detection of fungi in ears by immunofluorescence microscopy.
With manufacturing facilities in Pennsylvania, Rockland produces antibodies for western blotting, immunohistochemistry (IHC), immunofluorescence microscopy, ELISA and FLOW cytometry.
Slides were examined under ultraviolet light, and sera that had fluorescence on indirect immunofluorescence microscopy were carefully classified.
We then employed indirect immunofluorescence microscopy to examine localization of galectin-3 on HT-29,MSC-1 ,ASC-17D and MA-10 cells.
21,23-27] Like uncomplicated deposits, this microangiopathy primarily involves afferent arterioles, and large amounts of immunoglobulin and complement are found in arteriolar walls by immunofluorescence microscopy.
Commonly used laboratory methods for the detection of autoantibodies in the serum include indirect immunofluorescence microscopy and immunochemical assays (5).
Based on the findings of light and immunofluorescence microscopy and the presence of the fibrillary deposits on electron microscopy, a diagnosis of fibrillary GN was rendered.
With manufacturing facilities in Pennsylvania, Rockland produces over 10000 products designed for western blotting, immunohistochemistry (IHC), immunofluorescence microscopy, ELISA and FLOW cytometry.
Rockland Immunochemicals' antibodies and Antibody Based Tools[TM] are designed for individuals performing Western Blotting, Immunohistochemistry (IHC), Immunofluorescence Microscopy, ELISA, FLOW Cytomtery, ChIP experiments, as well as other immunodetection or immunofluorescent techniques.