Immunofluorescence

(redirected from immunofluorescent)
Also found in: Dictionary, Thesaurus, Medical.

Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
The results of immunofluorescent staining with the DAZL, SCP3, and TNP1 antibodies are shown in Table 2 and Figure 3.
prowazekii include enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent antibody assay, and indirect immunoperoxidase antibody assay.
Immunofluorescent studies: Intensity of fluorescence observed in tissues increased with the progression of disease, reached to peak at 8th DPI and then started subsiding (Table III).
To confirm its application as a diagnostic tool, indirect immunofluorescent staining method was performed on M.
For immunofluorescent studies, rejuvenated cells (see above) were allowed to attach overnight to coverslips.
The diagnosis of PCP was confirmed by immunofluorescent testing (Axis; Shield Diagnostics Ltd, UK) on the ETA samples, which were also negative for routine bacterial pathogens and TB.
However, suckling mice inoculation for virus isolation, a laborious and time consuming technique which requires many passages has been replaced by recent techniques such as immunofluorescent assay using serotype specific monoclonal antibodies.
Along with the response to RA, there was an associated increase in levels of involucrin and filaggrin at the protein level as demonstrated with immunofluorescent labelling.
Complement fixation, ELISA and immunofluorescent assays are commonly employed for this purpose.
canis (blood smears and nested PCR) and serological tests (Dot-ELISA and Indirect Immunofluorescent Antibody Test - IFAT), and analyzed clinical and hematological signs of dogs suspected of ehrlichiosis.
At ASVL, viral cytopathic effect was observed in viral culture cells from the specimen collected from the woman on October 10; however, immunofluorescent antibody staining was negative for herpes simplex virus (HSV).