(2007) Evaluation of immunoturbidimetric specific protein methods using the Architect ci8200: comparison with immunonephelometry
. Annals of Clinical Biochemistry 44, 52936.
The most common method of measuring IgG subclasses (IgG1-4) is immunonephelometry
(IN)7, of which there are 2 major vendors in North America: Binding Site[TM] IN (BSIN) and Siemens[TM] IN (SIN).
Marking of C-reactive protein concentration is based on the immune reaction of monovalent antibodies against CRP using the methods of radial immunodiffusion, immunoelectrophoresis, immunonephelometry
, radioimmunoassay, and enzyme immunoassay.
Quantitative estimation of serum hypersensitive C-reactive protein (hs-CRP) was done by means of particle enhanced immunonephelometry
using BN system (Dade Behring, USA).
Serum high-sensitivity C-reactive protein (hsCRP) was measured with particle-enhanced immunonephelometry
(Dade Behring, Marburg, Germany).
IgG4 was measured by immunonephelometry
using a Siemens BN II nephelometer, and serum sIL-2R levels were determined with ELISA (Diaclone, Besancon Cedex, France) according to manufacturer instructions in pg/ml.
Serum levels of IgG, IgA, and IgM were determined by immunonephelometry
(Immage 800, Beckman-Coulter, Brea, CA, USA).
High-sensitivity C-reactive protein was determined by immunonephelometry
(CardioPhase, Dade Behring, USA).
The serum concentrations of CRP were determined with immunonephelometry
, and ESR was measured using the traditional Westergren method.
To determine total IgE antibodies, the samples were analyzed within 24 hours by immunonephelometry
(Nephelometer Dade Behring, Marburg, Germany).
Total high-sensitivity C-reactive protein (hsCRP) levels were determined by immunonephelometry
on a BN ProSpec nephelometer (Dade Behring, Marburg, Hesse, Germany), according to the manufacturer method.
Automated high-sensitivity CRP measurement concentration was performed using particle-enhanced immunonephelometry
with an image analyzer.