immunoprecipitation


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immunoprecipitation

[‚im·yə·nō·prə‚sip·ə′tā·shən]
(immunology)
A protein purification method which involves the formation of an antibody-protein complex to separate out the protein of interest.
References in periodicals archive ?
The amount of ubiquitin protein combined to p27 was determined using an immunoprecipitation assay.
DRC, Democratic Republic of the Congo; EBOV, Ebola virus; LIPS, luciferase immunoprecipitation system; NA, not applicable; neut, neutralization assay; ROC, Republic of the Congo; +, positive.
(e) Immunoprecipitation using anti-BRK antibody and subsequent analysis of BRK, IRS-4 and phospho-IGF-1 receptor in samples from normal (N) and tumoural (T) tissues.
Using the KLF5 antibody, we were able to pull down the third and fourth KLF5 binding sequences from the MDM2 gene in chromatin immunoprecipitation assay, which was about the predicted size of 190 bp (Figure 5(c)).
Data shown are cropped images from one immunoprecipitation gel and one lysate gel and are representative of three individual experiments.
Immunoprecipitation assay suggests a functional overlapping between the two isoforms, JNK2 and JNK3.
(b) Immunoprecipitation and immunoblot analysis of the interaction between IKK[gamma] and SUMO1 or SUMO2/3 induced by high glucose was reversed by siRNA-PIASy-3.
Immunoprecipitation. Immunoprecipitations were carried out using the Direct Immunoprecipitation Kit (Pierce, Rockford, IL, USA) according to the manufacturer's instructions.
(b) Chromatin immunoprecipitation DNA was isolated with an anti-Bach1 antibody or with a Control IgG antibody, and then quantitative real-time RT-PCR analyses were performed with primer sequences from the indicated regions of the VEGF promoter in HUVECs (n = 4; * P < 0.05 versus Control IgG).
Since TAK1 is upstream of the MAPK/AP-1 pathway, a further validation experiment was carried out by immunoprecipitation analysis.
Among them, one of these host proteins, identified as porcine STK3 (NCBI reference sequence GACC01000309.1), was selected for further immunoprecipitation analysis.
Chromatin immunoprecipitation (ChIP) assay indicated that sinapine inhibited binding of the transcription factor NF-[kappa]B to the MDR1 promoter.