immunoprecipitation


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immunoprecipitation

[‚im·yə·nō·prə‚sip·ə′tā·shən]
(immunology)
A protein purification method which involves the formation of an antibody-protein complex to separate out the protein of interest.
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Abbreviations: FGFR, fibroblast growth factor receptor; FRS2[alpha], FGFR substrate protein-2[alpha]; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NF-[kappa]B, nuclear factor kappa-light-chain-enhancer of activated B cells; P-gp, P-glycoprotein; MDR, multidrug resistance; ChIP, Chromatin immunoprecipitation.
Immunoprecipitation of TP53 in MDA-MB-468 and MCF7 cell lysates and blotting for RAD51 revealed that mutated TP53 in MDA-MB-468 cell line was not able to bind RAD51.
Immunoprecipitation is a powerful tool to investigate protein-protein interactions and study, for example, the effects of alcohol on certain proteins and protein complexes and on the proteome of certain alcohol-sensitive cells.
Abbreviations Anti-Tyr(P): Anti-phosphotyrosine antibody ERK: Extracellular signal regulated kinase FAK: Focal adhesion kinase Hx: Hypoxanthine IP: Immunoprecipitation Resv: Resveratrol ROS: Reactive oxygen species WB: Western blot XO: Xanthine oxidase.
7- day mouse testes were used in Chromatin immunoprecipitation for their high rate of spermatogonia.
For protein interactions, useful approaches include immunoprecipitation (direct or of tagged proteins), followed by mass spectrometric studies, as well as protein arrays and indirect methods, such as yeast two-hybrid analyses.
We then performed a ChIP assay using the Chromatin Immunoprecipitation Assay kit (Upstate, Charlottesville, VA, USA) according to the manufacturer's instructions.
The final chapter explains chromatin immunoprecipitation to analyze histone modifications and chromatin-binding regulatory proteins.
This edition contains several new chapters on chromatin immunoprecipitation and other in vivo technologies, and new chapters on topological studies, photo crosslinking, FRET, and imaging techniques.
Binding of IK to the VPAC1 promoter was queried using chromatin immunoprecipitation (CHIP).
Caveolae were obtained using detergent (1% TritonX-100) and detergent-free methods as well as by immunoprecipitation with caveolin-3, a marker protein for caveolae.