immunoassay

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Immunoassay

An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay

immunoassay

[‚im·yə·nō′a‚sāy]
(immunology)
A laboratory detection method that uses antibodies to react with specific substances.
References in periodicals archive ?
Diffuse Cytokeratin 19 immunoreactivity was found in 93.
In the vehicle-ischemia group, SOD1 immunoreactivity in neurons of the stratum pyramidale decreased with time after TGCI, showing about 79% of the vehicle-sham group 2 days after TGCI and about 43% of the vehicle-sham group 5 days after TGCI [Figure 4]c, [Figure 4]e, and [Figure 4]m.
Thus we assume that the significant decrease in I-T-C recovery measured by the TnI19C7-TnI560 assay and the shift of I-T-C immunoreactivity peak in GF studies in some plasma samples are related to the binding of I-T-C to the specific autoantibodies fraction.
Immunoreactivity and amino acid content of fermented soybean products.
Microinjections of ET-1 Induced Progressive Impairment of MBP Immunoreactivity in the Ischemic Striatum.
The number of subjects was small and the immunoreactivity in patients could not be correlated with the treatment response.
22) Loss of immunoreactivity to SDHB protein has been seen in paraganglioma/pheochromocytoma with SDHA, SDHB, SDHC, SDHD, or SDHAF2 mutations, which presumably results from destabilization and degradation of the SDH complex, which is assembled from 4 separately coded subunits, when any of those subunits is mutated.
Progesterone receptor immunoreactivity was found exclusively in the nucleus of neurons for all ovarian development stages (moderately positive; Table 2).
In the osteoclastic giant cell leiomyosarcoma of kidney, you should have positive immunoreactivity with smooth muscle actin, desmin and pancytokeratin.
At about 60% of development, immunoreactivity to TPep antibodies can be seen in the pedal ganglia early in development.
Maximum muscle damage, IL-6 immunoreactivity and oxidant levels emerged one day after exhaustion in both trained and untrained groups.

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