immunoassay

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Immunoassay

An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay

immunoassay

[‚im·yə·nō′a‚sāy]
(immunology)
A laboratory detection method that uses antibodies to react with specific substances.
References in periodicals archive ?
The immunoreactivity of VEGF in the luminal and glandular layers of the endometrium was overall stronger than that in the stroma where the VEGF expression differed significantly between the phases of the cycle.
Conclusive results could not be determined because of minimal immunoreactivity (less than 1% of the cells).
F: Immunohistochemically positive immunoreactivity with histiocyte marker CD68 in multinuclear giant cells (immunostains, original magnification x400).
For example, diffuse immunoreactivity for p16 provides 65% sensitivity and 82% specificity for the diagnosis of ESC; diffuse immunoreactivity for p53 provides 55% sensitivity and 95% specificity of diagnosis of ESC.
In this study, muscular structures and IL-6 immunoreactivity in the control group were found to be normal (Figure 1,2).
Evaluation of expression of Orexin R1/2: The specific cytoplasmic immunoreactivity of Orexin R1/2 was semiquantitativeley for intensity and distribution on a grading scale as follows: Intensity of staining: no staining as 0; weak staining as 1 and strong staining as 2; Distribution of staining: no cells, less than 5% positive cells, 5-50% of cells and more than 50% of cells as 0, 1, 2 and 3, respectively.
Site-specific modification of the antibody is sometimes required when this type of random DOTA coupling results in loss of immunoreactivity attributable to the presence of a lysine residue in the antigen binding epitope.
Subsequently biopsy specimens obtained from labial glands were tested for AQP-5 expression and immunoreactivity using an AQP-5 ELISA kit and immunohistochemistry.
Vimentin immunoreactivity (IR) was observed at the zone of the optic chiasm and the third ventricle of hypothalamus, in the supra optic nuclei (SO) and optic chiasm (OC) (Fig.

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