in situ hybridization


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Related to in situ hybridization: Fluorescent in situ hybridization

in situ hybridization

[‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.
References in periodicals archive ?
In Japan, Fluorescence In Situ Hybridization (FISH) will reach a market size of US$42.1 Thousand by the close of the analysis period.
Human epidermal growth factor receptor 2 (HER2) test results distribution applying 2007, 2013, and 2018 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines on a series of 880 patients with invasive breast cancer selected for in situ hybridization analysis performed with dual-probe US Food and Drug Administration--approved fluorescent in situ hybridization assay (PathVysion, Abbott, Abbott Park, IL).
Comparison of immunohistochemical and fluorescence in situ hybridization assessment for HER-2/neu status in Taiwanese breast cancer patients.
Test Source Tests Positive Sensitivity performed (n) tests, n Cytology Bladder 354 72 59.3 UUT 379 151 74.6 FISH Bladder 264 46 52.9 UUT 171 90 79.0 NMP22 Bladder 240 164 62.5 UUT 40 38 100.0 uCyt+ Bladder 181 57 50.0 UUT 10 4 100.0 Test Specificity PPV NPV total Cytology 82.9 22.2 96.1 66.6 29.1 93.4 FISH 85.0 19.6 96.3 50.7 16.7 95.1 NMP22 31.3 6.1 92.1 5.9 15.8 100.0 uCyt+ 69.8 10.5 95.2 66.7 25.0 100.0 FISH = fluorescence in situ hybridization; NMP22 = nuclear matrix protein 22; uCyt+ = immunocytology; PPV/NPV = positive/negative predictive value.
Structural rearrangements, evolution of chromosome, and phylogenetic as well as genomic relationships can be studied extensively by modern cytogenetic techniques, that is, in situ hybridization by fluorescent and genomic probes (FISH and GISH) [11,12].
Bright-field HER2 dual in situ hybridization (DISH) assay vs fluorescence in situ hybridization (FISH): focused study of immunohistochemical 2+ cases.
Rapid diagnostic of bacterial meningitis by real-time PCR and fluorescent in situ hybridization. J Clin Microbiol 2005; 43: 3390-3397.
Maize chromosomesas Maize identified by chromosome SSR markers microsatellites markers 1 p-bnlg176, p-bng1671 1 2 p-bnlg1018,p-bnlg1887 2 3 p-bnlg1447, p-bnlg1350 3 4 p-umc1017, p-bnlg1019 4 5 p-bnlg1063, p-bnlg389 5 6 p-bnlg249, p-nc009 one chromosome substituted 7 p-bnlg1792, p-bnlg1247 7 8 p-bnlg1194, p-bnlg1863 8 9 p-bnlg279, p-bnlg1810 9 10 p-phi063, p-phi071 chromosomes substituted Abbreviations: DAPI, 4',6-diamidino-2-phenylindole; GISH, genomic in situ hybridization; McGISH, multicolor genomic in situ hybridization; PBS, phenobarbital sodium; PCR, polymerase chain reaction: SSC, standard saline citrate: SSR, simple-sequence repeat.
should offer more convincing evidence from images of viral particles in cells by electron microscope or in situ hybridization. Therefore, we think the CPE that Audelo-del-Valle et al.
Partial tetrasomy of chromosome 22 was confirmed by fluorescent in situ hybridization. To our knowledge, this" is the first report of such an occurrence in one family.
The company received United States Patent 6,277,569 B1, "Methods for Multiple Direct Label Probe Detection of Multiple Chromosomes or Regions thereof by In Situ Hybridization".