in situ hybridization(redirected from in situ nucleic acid hybridization)
Also found in: Medical.
in situ hybridization[‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.