The oxidation rates for incubations 1, 2, 6, 7, and 8, and ATP production ranged from 19% to 41% of the mean control value, whereas the oxidation rate of incubation 9 was strongly decreased (9% of the mean control value).
In all patients, the oxidation rates in incubations 1, 2, and 4 and the ATP production were decreased, with a normal incubation 2/incubation 1 ratio.
All incubations were performed in the presence of ADP and as described in the Materials and Methods section.
Glass incubation vials (20 mL) with injection caps and rubber septa, hydroxide of hyamine 10-X (hyamine), and Insta Fluor were from Packard BioScience.
Patient 15, with a complex III deficiency attributable to a mutation in the MT-CYB gene, had decreased substrate oxidation rates and disturbed incubation 1/incubation 2, incubation 7/incubation 6, and incubation 7/incubation 8 ratios.
The incubation period is primarily affected by the initial egg mass and speed of embryonic development (Deeming 2002).
Prolonged incubation has been recorded for a number of bird species (Holcomb 1970, Sutcliffe 1982, Margalida et al.
The White Stork has biological attributes thought to favor prolonged incubation (but not supported by empirical data).
I also describe abnormal behavior of the breeding pair which may help explain the prolonged incubation.
The average incubation period is 32 (29-34) days and usually starts with the second egg.