The comparison of indirect immunofluorescence
, enzyme immunoassay and western blot methods for the detection of antinuclear antibodies.
Phenotypic profile of lymphocytes of peripheral blood was investigated by indirect immunofluorescence
on flow cytometry in "FacsCalibur" (Becton Dickenson/USA) using monoclonal antibodies to surface antigens of lymphocytes (by method of A.
ANA, ASMA, AMA (anti mitochondrial antibody), anti-LKM, anti GPC (anti gastric parietal cell antibody) and antithyroid microsomal antibodies were tested by indirect immunofluorescence
(IIF) on tissue sections of rat liver, kidney and stomach and human thyroid using a serum dilution of 1:10.
Detection of Em A using indirect immunofluorescence
is considered the reference standard for CD-specific antibodies due to its unsurpassed sensitivity and specificity.
Using indirect immunofluorescence
test antisperm antibodies were detected in the serum of 64.4% of the infertile women (64.4%).
assay (IFA) technique: IFA was carried out with antiserum raised in adult mouse as described earlier (5).
gondii was investigated by indirect immunofluorescence
, considering 1:16 dilution as the cut-off, (IFAT; CAMARGO, 1964) in serum obtained from all the dogs two days prior to inoculation, on post inoculation day (PID) 7 and weekly up to PID 70.
Subsequently, indirect immunofluorescence
assays were conducted with mAb (60.11, anti-gC1qR) to assess gC1qR distribution and expression.
For the detection of C4d, indirect immunofluorescence
was employed, using biotinylated mouse anti-human monoclonal antibody and a FITC-conjugated rabbit anti-mouse IgG antibody (Biogenesis, Poole, England).
The samples were analyzed for antinuclear antibodies (ANAs) using a commercially available indirect immunofluorescence
The clinical differential diagnosis of OLP includes the autoimmune bullous diseases pemphigus vulgaris, mucous membrane pemphigoid, dermatitis herpetiformis, and linear immuno-globulin A disease, which can all be excluded with direct and indirect immunofluorescence
We then employed indirect immunofluorescence
microscopy to examine localization of galectin-3 on HT-29,MSC-1 ,ASC-17D and MA-10 cells.