Energy metabolism of the trophectoderm and inner cell mass
of the mouse blastocyst.
Table 1: Advantages and disadvantages of inner cell mass
(ICM) isolation from human embryos.
Trounson, "Simplified technique for differential staining of inner cell mass
and trophectoderm cells of mouse and bovine blastocysts," Reproductive BioMedicine Online, vol.
After 6 h in the culture, the number of expanded blastocysts (with intact zona pellucida and refraction The double dye technique allowed a reliable and obvious distinction between the pink trophoectoderm nuclei and intact inner cell mass
in which the nuclei appear blue.
The normal allocation and integrity of inner cell mass
between IMO produced blastocyst and SOFaaBSA alone after differential staining was significantly different (Figure 3).
Our finding was in contrast to what was reported in bovine in which the IVM medium that was supplemented with serum resulted in blastocysts with a larger number of cells allocated to the inner cell mass
(ICM) compared with those supplemented with fatty acid-free serum albumin (9).
By the time of hatching, the inner cell mass
has differentiated into an outer layer of cells called the primitive endoderm (or hypoblast) and an inner layer of cells called the primitive ectoderm (or epiblast).
B-myb is required for inner cell mass
formation at an early stage of development.
At this point, an embryonic stem cell line could be made by transferring cells from the inner cell mass
to a culture dish and growing them in a medium that provides support and nutrients.
In 1981, it was proved that mouse Embryonic Stem (ES) cells are derived from the inner cell mass
It develops into a blastocyst consisting of two groups of cells: an inner cell mass
of embryonic stern-cells destined to form an embryo and peripheral cells destined to form the non-maternal parts of the placenta and membranes.
In addition, the Patent Office has also recently issued notices of allowance for related patent applications in the portfolio, including United States Patent Applications 09/650,194 and United States 09/989,126 directed to methods of cloning ungulate animals, fetuses and embryos, and of producing mammalian inner cell mass