Energy metabolism of the trophectoderm and
inner cell mass of the mouse blastocyst.
Table 1: Advantages and disadvantages of
inner cell mass (ICM) isolation from human embryos.
Trounson, "Simplified technique for differential staining of
inner cell mass and trophectoderm cells of mouse and bovine blastocysts," Reproductive BioMedicine Online, vol.
After 6 h in the culture, the number of expanded blastocysts (with intact zona pellucida and refraction The double dye technique allowed a reliable and obvious distinction between the pink trophoectoderm nuclei and intact
inner cell mass in which the nuclei appear blue.
The normal allocation and integrity of
inner cell mass between IMO produced blastocyst and SOFaaBSA alone after differential staining was significantly different (Figure 3).
Our finding was in contrast to what was reported in bovine in which the IVM medium that was supplemented with serum resulted in blastocysts with a larger number of cells allocated to the
inner cell mass (ICM) compared with those supplemented with fatty acid-free serum albumin (9).
By the time of hatching, the
inner cell mass has differentiated into an outer layer of cells called the primitive endoderm (or hypoblast) and an inner layer of cells called the primitive ectoderm (or epiblast).
B-myb is required for
inner cell mass formation at an early stage of development.
At this point, an embryonic stem cell line could be made by transferring cells from the
inner cell mass to a culture dish and growing them in a medium that provides support and nutrients.
In 1981, it was proved that mouse Embryonic Stem (ES) cells are derived from the
inner cell mass of blastocysts.
It develops into a blastocyst consisting of two groups of cells: an
inner cell mass of embryonic stern-cells destined to form an embryo and peripheral cells destined to form the non-maternal parts of the placenta and membranes.
In addition, the Patent Office has also recently issued notices of allowance for related patent applications in the portfolio, including United States Patent Applications 09/650,194 and United States 09/989,126 directed to methods of cloning ungulate animals, fetuses and embryos, and of producing mammalian
inner cell mass cells.