The effects of inoculum
sizes on invertase production by P.
effects noted in this study with ceftazidime-avibactam MICs are not surprising given that inoculum
effects have been previously documented with ceftazidime MIC testing, particularly against [beta]-lactamase producing strains [5-7].
The most important variables for EPS production were inoculum
concentration, followed by time of growth, and also the interaction among these variables according the p-values indicated in Table 2.
The first question concerns whether the Pr[P.sup.Sc] recovered on first passage represents the residual inoculum
rather than de novo Pr[P.sup.Sc] generated by conversion of the host's Pr[P.sup.C].
The three studied variables were coded as: moisture content (A), inoculums
size (B) and temperature (C).
Francesca Hand (Doug Gubler's group), in conjunction with local crop production advisors, tested the inoculum
detection technology in Fresno, Napa, Monterey, Sacramento, Solano and Sonoma counties of California in 2010-12.
Substrate to inoculum
ratios (S/I ratios) for BMP assay cultures were prepared based on the VS content of substrate and inoculum
, as shown in Table 2.
Microorganism, culture maintenance, inoculum
preparation and microorganism manipulation
Combine Mueller-Hinton broth and preservative solution to achieve a final volume of 1 mL, keeping in mind that the inoculum
will be added in step four.
It will also be important to limit opportunities for introduction of inoculum
with soil or transplants.
These include postinflammatory hyperpigmentation, inflammatory nodules at inoculums
site.5,6 Inflammatory nodule may be most likely due to failure in maintaining procedure sterility, as in one of our patients who had genital lesion.