Experimental configurations for biodegradation evaluation Treatments Inoculum
Substrate (g [VSS.L.sup.-1]) (2 g [DQO.L.sup.-1]) 1 2 Bovine rumen Cassava wastewater 2 2 Bovine rumen Cassava wastewater Treatments Temperature ([degrees]C) 1 32 2 39 Table 3.
The preliminary in vitro investigations revealed that the aqueous extract of Hedychium spicatum has strong fungicidal efficacy at low MIC against Rhizoctonia solani, inhibiting heavy doses of inoculums
, long shelf life, thermostable and having broad antifungal spectrum.
For citric acid, as the table shows, the MIC was 0.625% for inoculums
of 10, [10.sup.2], and [10.sup.3] CFU/ml.
The phylum Firmicutes (34% (SS) and 13% (IGBS)) was represented by Clostridia and Bacilli classes in both inoculums
(Figures 1(a) and 1(b)).
Two (2.4%) patients had infection on inoculation site, of which one had inoculum
taken from genital lesion.
When the homogenate was used at 4.76% (v/v) inoculum
level, the cultures produced biomass and exopolysaccharide of 15.75 and 0.54 g/L, respectively; these values were 129.26 and 45.95% higher than that of the control culture using a pellet-type inoculum
Operational variables as pH and initial inoculum
size as well as supplementation of industrial media with yeast extract, and tryptone were evaluated by means of experimental methodology.
The three studied variables were coded as: moisture content (A), inoculums
size (B) and temperature (C).
Microorganism and inoculum
preparation: Fungal strain used in this study was obtained from the Fungal Bank University of The Punjab Lahore.
S/I ratio can be expressed as the amount of VS in the substrate per the amount of VS or amount of volatile suspended solid (VSS) originating from inoculum
. Chynoweth et al.
After decoding these strings to real values through linear mapping, the optimum values of process parameters were seen to be equal to 38.82[degrees]C, 10.285%, 9.392%, 7.32, and 2.995 h for fermentation variables such as temperature, oil concentration, inoculums
size, pH, and incubation time, respectively.
The effect of different concentrations (1%, 2%, 3%, 4%, and 5%) of the substrate was carried out and the effect of different inoculum
sizes (1%, 2%, 3%, 4%, and 5%) of the substrate was also studied.