We focused on the region of the gene where the IS407A
insertion sequence apparently was introduced to create the B.
The
insertion sequence IS6110 is one of the most common DNA target sequence used for diagnosis and fingerprinting of M.
GP, [beta]-glucuronidase-positive; IS,
insertion sequence.
Since then, mcr-1 has been identified on plasmids of various incompatibility (Inc) types associated with 0, 1, or 2 copies of the
insertion sequence (IS) ISApl1 (2), and it has been identified in other species (most notably Salmonella spp.) and in several other countries (3), not including Australia.
rmtE1 is bound by an IS CR20-like element and an IS 1294-like
insertion sequence. This immediate unit is identical to that found in pYDC637.
meningitidis adhesin A variant had an
insertion sequence that disrupted this gene, as described for ET-15 meningococci (10).
Moreover, the genetic environment of fosA3 was assessed by PCR mapping and sequencing (2,6), showing a composite transposon containing an
insertion sequence 26 323 bp upstream fosA3; the orfl, orf2, and orf3 genes (homologous to regulatory ones in K.
The LV143 strain was positive for the
insertion sequence ISEcp1, associated with [bla.sub.CTX-M-15] (Figure), and was negative for the class 1 integron (data not shown).
qPCR targeting the repeated
insertion sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8).
Analysis by mycobacterial interspersed repetitive unit-variable number tandem repeats (15) identified 2 strain variants in our sample set (Table 2); IS6110 DNA fingerprint analysis (12) of isolate MK172 showed it to contain 21 copies of the IS6110
insertion sequence element (Figure 1).
sonnei (Ss046) it is inactivated by a frameshift mutation and an
insertion sequence, respectively (12).
Cloning and sequencing of the genetic context of the [bla.sub.NDM-1] in the first isolate showed that transposon Tn125 was truncated at its 3'-end extremity by
insertion sequence ISA-ba14, giving rise to a truncated Tn125 ([DELTA]Tn125).