We focused on the region of the gene where the IS407A insertion sequence
apparently was introduced to create the B.
The insertion sequence
IS6110 is one of the most common DNA target sequence used for diagnosis and fingerprinting of M.
GP, [beta]-glucuronidase-positive; IS, insertion sequence
Since then, mcr-1 has been identified on plasmids of various incompatibility (Inc) types associated with 0, 1, or 2 copies of the insertion sequence
(IS) ISApl1 (2), and it has been identified in other species (most notably Salmonella spp.) and in several other countries (3), not including Australia.
rmtE1 is bound by an IS CR20-like element and an IS 1294-like insertion sequence
. This immediate unit is identical to that found in pYDC637.
meningitidis adhesin A variant had an insertion sequence
that disrupted this gene, as described for ET-15 meningococci (10).
Moreover, the genetic environment of fosA3 was assessed by PCR mapping and sequencing (2,6), showing a composite transposon containing an insertion sequence
26 323 bp upstream fosA3; the orfl, orf2, and orf3 genes (homologous to regulatory ones in K.
The LV143 strain was positive for the insertion sequence
ISEcp1, associated with [bla.sub.CTX-M-15] (Figure), and was negative for the class 1 integron (data not shown).
qPCR targeting the repeated insertion sequence
IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8).
Analysis by mycobacterial interspersed repetitive unit-variable number tandem repeats (15) identified 2 strain variants in our sample set (Table 2); IS6110 DNA fingerprint analysis (12) of isolate MK172 showed it to contain 21 copies of the IS6110 insertion sequence
element (Figure 1).
sonnei (Ss046) it is inactivated by a frameshift mutation and an insertion sequence
, respectively (12).
Cloning and sequencing of the genetic context of the [bla.sub.NDM-1] in the first isolate showed that transposon Tn125 was truncated at its 3'-end extremity by insertion sequence
ISA-ba14, giving rise to a truncated Tn125 ([DELTA]Tn125).