intercalating agent

intercalating agent

[‚in·tər′ka‚lād·iŋ ‚ā·jənt]
(cell and molecular biology)
A chemical substance that can insert itself between base pairs in a deoxyribonucleic acid molecule.
References in periodicals archive ?
An intercalating agent solution/dispersion (0.0688 mol/L), in the case of ZHN, or (0.107 mol/L), in the case of CHN, was prepared.
In this process, ZHN was added to an intercalating agent solution and this mixture was vigorously stirred for three days, after which the exchanged layered hydroxide salt suspension was centrifuged at 4000 rpm for 10 minutes and washed with deionized water.
Amplification of cDNA sequences was performed using the PCR Master Mix kit (Applied Biosystems) containing the SYBR Green intercalating agent, a fluorescent molecule that intercalates into double-stranded DNA after initial denaturation at 95[degrees]C for 15 s, followed by 60[degrees]C for 1 min.
During agarose gel electrophoresis, plasmid pBR322 the drug-free DNA is negatively supercoiled and migrates through the gel as a single band, but its profile changes significantly in the presence of an intercalating agent (EtBr).
This intercalating agent proved to be not only more secure in its use, but also much more sensitive than the ethidium bromide, presenting better linear relationship between the amount of PCR products and the fluorescence intensity (6).
Pillarisation and intercalation process of the clay was carried out by mixing bentonite, pillaring agent solution and intercalating agent (hexadecyltrimethylammonium-bromide; HDTMA-Br) with ratio [gram bentonite/ volume of solution] = 1 gram/50 mL.
Single-stranded DNA was immobilized by covalent binding to HEG linkers on fused silica optical fibres, and hybridization was detected using the fluorescent intercalating agent ethidium bromide.
Doxorubicin (0.5 g/L) was used as a typical intercalating agent with major binding (100%) capability.
All the LDHs were prepared by the conventional coprecipitation method [20], 0.03 mol Mg[(N[O.sub.3]).sup.2]-6[H.sub.2]O and 0.01 mol Al[(N[O.sub.3]).sup.3] x 9[H.sub.2]O with 0.01 mol intercalating agent (sodium dodecylbenzenesulfonate or sodium stearate) were dissolved in 300 mL deionized water, the pH value was adjusted to approximately 10 with a 1 mol [L.sup.-1] NaOH aqueous solution.
Where 88 meg/100 g represents the CEC value per 100 g of clay, and 1.5 (>1) indicates the excess amount of CTAB (intercalating agent).
Someya and Shibata (24) studied the morphology and thermal and mechanical properties of VER-clay nanocomposites with clay intercalating agent. They concluded that the intercalating agent has a significant effect on the intercalation and exfoliation behavior of clay into the resin matrix.
In this case, the barrier properties improvement merely depends on the diffusion, rather than solubility (Table 4): carvacrol, acting as an intercalating agent, promotes swelling of the clay platelets, thus increasing the average aspect ratio of the inclusions.