enzyme production was only shown by strains 2AS, 2AW and 3W.
RSM was applied to optimize the production of inulinase
(dependent variable) using yeast extract ([X.sub.1]) as the source of nitrogen and agave syrup ([X.sub.2]) as the carbon source (independent variables).
Awad, "Immobilized inulinase
on grafted alginate beads prepared by the onestep and the two-steps methods," Industrial and Engineering Chemistry Research, vol.
Pandey, "Solid state fermentation for the synthesis of inulinase
from Staphylococcus sp.
Optimization of inulinase
production by Aspergillis niger using simplex and classical method.
These strains are Gram-negative, rod shaped, nonmotile with 1.5-2.0 um long, and 0.9 [micro]m width, the biochemical tests indicated that the strain RCRI4 is capable to produce amylase, lipase, DNase, inulinase
, pectinase, but is not capable to produce other hydrolytic enzymes.
biosynthesis using immobilized mycelium of Aspergillus niger.
In determining the total fructose content, fructans were hydrolyzed at 60 [degrees] C for 30 min by 12.5 U m[L.sup.-1] of the inulinase
Novozyme SP 230 (Novo Industries, Copenhagen, Denmark) as described by Klaushofer and Wolfslehner (1983).
The process uses comminuted chicory roots or other plants, demineralizing the material and treating with an enzyme called inulinase
to remove the small sugars.
Depolymerization of inulin involves the action of enzymes namely, inulinase
. They can be divided into exo-inulinases and endo-inulinase .