ISSR-PCR markers used to analyze polymorphism of DNA fragments flanked by inverted repeats
of microsatellite loci based upon the standard protocol (Zietkiewiez et al., 1994).
They exhibit a conserved quadripartite structure consisting of one large single copy (LSC) region, one small single copy (SSC) region, and two copies of inverted repeats
The palindrome in EMBOSS 6.3.1  (http://mobyle .pasteur.fr/cgi-bin/portal.py?#forms::palindrome) was used to identify inverted repeats
in the Oesophagostomum species.
ISTN3X; 96% identical), comprising one of Tn3-like inverted repeats
and putative coding regions for transposase, resolvase (also called repressor), and ampicillin resistance.
Different oligonucleotides with inverted repeats
were designed to form hairpin loop structures to test efficiency of the sodium bisulfite reaction method.