Proteins in isoconcentration and isovolume were loaded on 4-12% NuPAGE BisTris gels (Invitrogen Corp., Carlsbad, CA, USA) or 4-15% Mini PROTEAN TG precast polyacrylamide gels (Bio-Rad Laboratories, Hercules, CA, USA) and then were transferred into PVDF membranes (Bio-Rad Laboratories).