isoelectric focusing


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Related to isoelectric focusing: isoelectric point

isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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The protein fractions showing considerable variations were subjected to isoelectric focusing.
ELECTROPHORESIS OF HUMAN SERUM PROTEINS FOLLOWING MYOCARDIAL INFARCTION
Quantification of hemoglobin A1c by capillary isoelectric focusing.
Low birth weight is not associated with type 2 diabetes in African American children in New Orleans
Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate.
Comparative studies on antibody detection using yeast phase lysate antigens prepared from virulent and avirulent isolates of Blastomyces dermatitidis
Quantification of hemoglobin variants by capillary isoelectric focusing.
Determinacion de la prevalencia de la hemoglobina S, C, D y G en recien nacidos de Buenaventura, Colombia
Detection of oligoclonal IgG bands (OB) in CSF by isoelectric focusing (IEF) is the most sensitive (approximately 95% in Western patients) laboratory test for MS and the best method to show local intrathecal IgG-synthesis (Figure 1).
Clinical usefulness of cerebrospinal fluid evaluation
Proteins migrate in isoelectric focusing gels to their neutral isoelectric point (pI), where the protein has zero net charge.
Introduction to proteomics
We fractionated cells by preparative isoelectric focusing in a pH gradient of 3-10 and bioassayed the resultant fractions in test diets to determine their effects on egg produc tion.
Improved fecundity in the predator Orius insidiosus (Hemiptera: Anthocoridae) with a partially purified nutritional factor from an insect cell line
The new system, which fully automates all processes from isoelectric focusing to polyacrylamide gel electrophoresis to detection, can perform two-dimensional electrophoresis of proteins in about one hour.
National Institute of Advanced Industrial Science and Technology (AIST), Others Co-develop High-speed Fully Automated 2-D Electrophoresis System for Protein Analysis
This protein was found to be present in numerous cell cultures so I purified to homogeneity by preparative isoelectric focusing and SDS polyacrylamide gel electrophoresis this protein from human embryonic kidney cells (i.
Comparison of the levels of the spicing factor PSF in individuals with dystonia
The technique gives simultaneous focusing and separation of differently charged analytes in a manner analogous to isoelectric focusing of proteins, but is much simpler to implement than isoelectric focusing and has the additional advantage of working with any charged analyte, rather than just proteins.
Temperature gradient focusing in microfluidic channels. (General Developments)
Isoelectric focusing (IEF) in agarose gels revealed species-specific protein profiles in the ten control species.
Biochemical identification of shark fins and fillets from the coastal fisheries in New Zealand

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