isoelectric focusing


Also found in: Dictionary, Thesaurus, Medical, Acronyms, Wikipedia.
Related to isoelectric focusing: isoelectric point

isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
Mentioned in ?
References in periodicals archive ?
Isoelectric focusing determined that the isoforms, named GST1 and GST2, have pI values of 4.5 and 6.2, respectively.
Characterization of affinity-purified isoforms of Acinetobacter calcoaceticus Y1 glutathione transferases
Each of the eluted protein was freeze dried and reconstituted in the buffer when used for isoelectric focusing. 10 uL solution D (10 % w/v sodiumdodecyle sulfate in 2.3 % w/v dithioerythreitol) was added to 60 uL eluted protein solution, mixed and heated at 95degC for 5 minutes.
ELECTROPHORESIS OF HUMAN SERUM PROTEINS FOLLOWING MYOCARDIAL INFARCTION
The optimized two-dimensional electrophoresis technology for ramie consists of the optimized TCA/acetone extraction method, protein purification using a four-fold volume of pre-cooled acetone after the cleavage reaction; isoelectric focusing for 50000 VH.
Establishment and Optimization of Two-dimensional Electrophoresis (2- DE) Technology for Proteomic Analysis of Ramie
For getting an idea about separation accuracy during different purification processes all fractions were tested with inhibitor activity by IEF technique which could be use also as a detection method according to isoelectric focusing point.
Isolation and characterization of trypsin inhibitors (Kunitz Soybean Trypsin Inhibitor, Bowman-Birk Inhibitor) in Soybean
Quantification of hemoglobin A1c by capillary isoelectric focusing. Annual meeting of the American Association of Clinical Chemistry, Atlanta, Ga, 1997, 1997.
Low birth weight is not associated with type 2 diabetes in African American children in New Orleans
Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate.
Comparative studies on antibody detection using yeast phase lysate antigens prepared from virulent and avirulent isolates of Blastomyces dermatitidis
This hemoglobin variant was detected by isoelectric focusing (IEF) moving just ahead of Hb A.
First observation of Hb Tunis [beta 124(H2) Pro>Ser] in Turkey / Turkiye'de gozlenen ilk Hb Tunis [beta 124(H2)Pro>Ser] olgusu
Quantification of hemoglobin variants by capillary isoelectric focusing. Clin Chem.
Determinacion de la prevalencia de la hemoglobina S, C, D y G en recien nacidos de Buenaventura, Colombia
* Detection of oligoclonal IgG bands in CSF by isoelectric focusing is an important part of the diagnostic process in MS (Class I evidence).
Clinical usefulness of cerebrospinal fluid evaluation
Capillary isoelectric focusing (clEF) is another accurate method where the sensitivity of detection of capillary electrophoresis is combined with the automated sampling and data acquisition of HPLC (5,6).
Evaluation of chromatographic & electrophoretic methods for diagnosis of the [beta]-thalassaemias
These labelled samples are then combined, run on an isoelectric focusing (IEF) gel in the first dimension, and separated by SDS-PAGE in the second dimension (Figure 2).
The advantages of 2-D Dige: this article discusses the key benefits of 2-D DIGE (two-dimensional fluorescence difference gel electrophoresis) compared with traditional techniques used to separate and analyse complex protein mixtures. 2-D DIGE is making major contributions to the value and breadth of 2-D electrophoresis applications in both pharmaceutical discovery and process development
Synthetic EPO has slightly different molecular forms to the natural EPO and detection methods include separating those forms into a characteristic pattern using a technique known as isoelectric focusing.
Race to keep pace with sports cheats; Expert verdict The Olympic Games begin on Friday and the world's analytical chemists will lead the battle to catch the drug cheats. ROGER JEWSBURY, Head of Department of Chemical and Biological Sciences in the School of Applied Sciences at the University of Huddersfield reports

Encyclopedia browser ?
Full browser ?