It was decided not to carry out the isoelectric precipitation
in the supernatant, which was conducted directly for lyophilization.
The use of bean protein isolates is increasing as a functional ingredients to improve the nutritional quality of the products .The most widely used procedure to prepare protein isolates is isoelectric precipitation
The isoelectric precipitation method was used for the recovery of respective protein isolates from defatted legume samples.
Earlier, Rangel et al., (2004) indicated that cowpea protein isolates (CPI) were obtained by isoelectric precipitation from defatted cowpea meal.
Then the super-natant fractions underwent isoelectric precipitation with 1 M Tris at pH 5.1 and 4.5, as previously reported with few modifications .
Trefoil factor 3 (TFF3) was purified from human breast milk by ammonium sulfate precipitation followed by isoelectric precipitation, gel filtration, and DEAE-Sepharose chromatography to ensure the removal of putative contaminating proteins (Figure 1, A and B).
Summary: The functional properties of sesame seed protein was investigated using reverse micelles system compared to the traditional procedure (alkali-solution and isoelectric precipitation).
Currently, protein is traditionally prepared by alkaline extraction and isoelectric precipitation. The sesame protein is prepared by alkaline extraction after the sesame oil extraction, the supernatant is centrifuged, and pH of the solution is adjusted to the isoelectric point to obtain protein precipitation.
 was used for the alkaline extraction- isoelectric precipitation production of proteins; the defatted cashew nut flour was extracted at room temperature (about 20[degrees]C) by using two different cashew nut powder to water ratios of 1:5, and 1:10.
This confirmed the findings of Damodaran  that most proteins are highly soluble at alkaline pH (7-9), and that protein extraction from plant sources is best carried out at this pH range, and the protein is best recovered from the extract by isoelectric precipitation at pH 4.5-4.8.
This was followed by the isoelectric precipitation
of the proteins at pH 5.3 to pH 5.5.
This may represent isoelectric precipitation
of protein (4) and include hemoglobin, red cells, and red cell debris.