By studying a virus responsible for feline leukemia, researchers at the Harvard School of Public Health in Boston and at Colorado State University in Fort Collins found that procedures used to separate viruses may fail to
isolate "acutely pathogenic" (disease-producing) virus strains while selecting for less virulent viruses.
The Photorhabdus
isolates recovered from nematodes and from the patient's hand were compared on the basis of nucleotide sequences of 2 housekeeping genes, glnA and gyrB.
Sequence analysis indicated that the new H5N1
isolate (A/duck/Laos/3295/2006) (GenBank accession no.
The sequences of our
isolates did not feature any codon deletions or insertions when compared with other CHIKV
isolates from Africa and Asia available in GenBank (4,6).
Only 8
isolates of this serotype have been reported worldwide, in 1974, 1985, 1986, 1987 (n = 2), 1998, and 2000 (n = 2).
This
isolate overproduced (6-fold) AcrA, which correlated with a higher ciprofloxacin MIC (16 mg/L).
DQ381540,
isolate IMTSSA/5163) and used in a BLAST search that identified O'nyong-nyong virus (ONNV, E value [6e.
Since the outbreak, 1 additional human
isolate of DT 104A was identified from a resident of the same neighboring region.
