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One clear isosbestic point that is observed at 685 nm (Figure 3(b)) proves that the phenomenon is due to some equilibrium processes leading to the formation of chemical intermediates between [beta]-carotene and the MnTTPCl/n-Au hybrid.
Moreover, the isosbestic point about 308 nm gives support to the DNA/Au@tiopronin complex formation.
An isosbestic point at 472 nm (Figure 1) supports this conclusion, since it indicates that only two molecular forms are present in the solution.
Excitation with the 360 nm filter (close to the Fura-2 isosbestic point) allowed observation of the cells' morphology and of the changes in the concentration of the dye, irrespective of the changes in [[[Ca.sup.2+]].sub.i], while the 360/380 nm ratio allowed visualization of the [[Ca.sup.2+]].sub.i] changes in the cytoplasm.
Isosbestic point. It is a specific wavelength at which two chemical species have the same molar absorptivity [epsilon] or more generally are linearly related.
We measured the absorbance at 540 nm (COHb) and 555 nm (isosbestic point) and calculated the percent COHb concentration.
Elucidation of a photoproduct was shown through an isosbestic point on UV-vis and through the absorhance of a photoproduct peak on the HPLC plots.
An isosbestic point at 575 nm also provided an evidence for the new Tm(III)-ASA complex formation.
For concentrations from 200 [micro]M and upwards, an orderly isosbestic point appears at 581.4 [+ or -] 0.1 nm (Figure 3(b)).
The spectra show a clear isosbestic point at 445 nm upon addition of [Hg.sup.2+] in 0.005 mol [L.sup.-1] HN[O.sub.3], demonstrating that the aggregation of AgNPs is directly related to the concentration of [Hg.sup.2+].