Table 2: Pipetting Scheme Tube Primary Antibody GAM-FITC PI (10 mg [mL.sup.-1]) 1 Cyclin A
isotypic 20 - 0.5 mL control-FITC [micro]L 2 Cyclin A-FITC 20 - 0.5 mL [micro]L 3 Cyclin B
isotypic 20 - 0.5 mL control-FITC [micro]L 4 Cyclin B 20 - 0.5 mL [micro]L 5 Cyclin D
isotypic 20 - 0.5 mL control-FITC [micro]L 6 Cyclin D 20 - 0.5 mL [micro]L 7 Cyclin E
isotypic 20 10 0.5 mL control (pure) [micro]L [micro]L 8 Cyclin E (pure) 20 10 0.5 mL [micro]L [micro]L 9 P21 (pure) 20 10 0.5 mL [micro]L [micro]L 10 P16
isotypic 20 - 0.5 mL control-FITC [micro]L 11 P16-FITC 20 - 0.5 mL [micro]L Assay
(i) E [??] P such that P' < E and [bar.E] is elementary abelian, with the
isotypic decomposition with respect to E, V = V [direct sum] xxx [direct sum] [V.sub.m], where m [greater than or equal to] [p.sup.l/3], and the summandsform a system of imprimitivity for P.
The possibility of a nonspecific signal was rejected, as it was not detected in the lanes corresponding to an immunoprecipitation with an irrelevant
isotypic antibody or without antibody.
To complete our proof of the infinitesimal rigidity of [Q.sub.4], it suffices to show that, if our section h belongs to an
isotypic component of C[infinity]([F.sub.C]), then it vanishes.
To ensure the specificity of the results, nonspecific antibody background binding was determined with the appropriately labeled
isotypic control immunoglobulin IgG.
Parallel with specific staining fluorochrome-conjugated
isotypic controls were run.
(1) In a model representation,
isotypic components are irreducible components, so projection operators map directly onto irreducible modules without being mixed up among multiple isomorphic copies of the same module.
It is convenient to consider the decomposition of M in
isotypic components as [k.sup.G]-module: M = [[cross product].sub.g[member of]G] M[g], where M[g] = [[delta].sub.g] x M.
Tubes were prepared with the following combinations after careful titrations of antibodies: IgGl-FITC/IgGl-PE (
isotypic control), CD45-FITC/CD34-PE, Rhodamine (Rhd)123/CD34-PE, CD34-FITC (for absolute counts), CD34-FITC/ HLA-DR-PE, CD34-FITC/CD38-PE, CD71-FITC/CD34-PE, CD9O-FITC/CD34-PE, and CD135-FITC/CD34-PE.
ZAP-70 expression in B-cell chronic lymphocytic leukemia: evaluation by external (
isotypic) or internal (T/NK cells) controls and correlation with IgV(H) mutations.
Relationship to other species: It is
isotypic with jamesite whose revised formula is [Pb.sub.2]Zn[[Fe.sup.3+].sub.2]([[Fe.sup.3+].sub.2.8][Zn.sub.1.2])[([ AsO.sub.4]).sub.4][(OH).sub.8][[(OH).sub.1.2][O.sub.0.8]].
In an additional experiment, the primary antibody was replaced with
isotypic mouse immunoglobulin IgG2b (Sigma) at the same concentration.