Table 2: Pipetting Scheme Tube Primary Antibody GAM-FITC PI (10 mg [mL.sup.-1]) 1 Cyclin A isotypic
20 - 0.5 mL control-FITC [micro]L 2 Cyclin A-FITC 20 - 0.5 mL [micro]L 3 Cyclin B isotypic
20 - 0.5 mL control-FITC [micro]L 4 Cyclin B 20 - 0.5 mL [micro]L 5 Cyclin D isotypic
20 - 0.5 mL control-FITC [micro]L 6 Cyclin D 20 - 0.5 mL [micro]L 7 Cyclin E isotypic
20 10 0.5 mL control (pure) [micro]L [micro]L 8 Cyclin E (pure) 20 10 0.5 mL [micro]L [micro]L 9 P21 (pure) 20 10 0.5 mL [micro]L [micro]L 10 P16 isotypic
20 - 0.5 mL control-FITC [micro]L 11 P16-FITC 20 - 0.5 mL [micro]L Assay
(i) E [??] P such that P' < E and [bar.E] is elementary abelian, with the isotypic
decomposition with respect to E, V = V [direct sum] xxx [direct sum] [V.sub.m], where m [greater than or equal to] [p.sup.l/3], and the summandsform a system of imprimitivity for P.
The possibility of a nonspecific signal was rejected, as it was not detected in the lanes corresponding to an immunoprecipitation with an irrelevant isotypic
antibody or without antibody.
To complete our proof of the infinitesimal rigidity of [Q.sub.4], it suffices to show that, if our section h belongs to an isotypic
component of C[infinity]([F.sub.C]), then it vanishes.
To ensure the specificity of the results, nonspecific antibody background binding was determined with the appropriately labeled isotypic
control immunoglobulin IgG.
Parallel with specific staining fluorochrome-conjugated isotypic
controls were run.
(1) In a model representation, isotypic
components are irreducible components, so projection operators map directly onto irreducible modules without being mixed up among multiple isomorphic copies of the same module.
It is convenient to consider the decomposition of M in isotypic
components as [k.sup.G]-module: M = [[cross product].sub.g[member of]G] M[g], where M[g] = [[delta].sub.g] x M.
Tubes were prepared with the following combinations after careful titrations of antibodies: IgGl-FITC/IgGl-PE (isotypic
control), CD45-FITC/CD34-PE, Rhodamine (Rhd)123/CD34-PE, CD34-FITC (for absolute counts), CD34-FITC/ HLA-DR-PE, CD34-FITC/CD38-PE, CD71-FITC/CD34-PE, CD9O-FITC/CD34-PE, and CD135-FITC/CD34-PE.
ZAP-70 expression in B-cell chronic lymphocytic leukemia: evaluation by external (isotypic
) or internal (T/NK cells) controls and correlation with IgV(H) mutations.
Relationship to other species: It is isotypic
with jamesite whose revised formula is [Pb.sub.2]Zn[[Fe.sup.3+].sub.2]([[Fe.sup.3+].sub.2.8][Zn.sub.1.2])[([ AsO.sub.4]).sub.4][(OH).sub.8][[(OH).sub.1.2][O.sub.0.8]].
In an additional experiment, the primary antibody was replaced with isotypic
mouse immunoglobulin IgG2b (Sigma) at the same concentration.