Male jirds of 8-10 weeks old were transplanted intraperitoneally with freshly isolated adult worms from peritoneal cavity of the jirds harboring 5-6 months old B.
Table 7 Antifilarial activity of Alnus nepalensis and DEC against Brugia malayi in jirds (Meriones unguicuIatus).
agent Mf motility A ND B ND C 144.83 D ND DEC-citrate 25.70 (d) Compound C exhibited promising in vitro adulticidal activity as evidenced by low LC100 and I[c.sub.50] values, therefore followed up for in vivo testing in jird model harboring transplanted adult worms of B.
The prevalence by animal species varied from 9.7% (3/31) in the Cairo spiny mouse (Acomys cahirinus) to 100% (10/10) in the bushy-tailed jird (Sekeetamys calurus).
Host animals A B C Daurian ground squirrel 1 4962 4963 Siberian chipmunk 1 4965 4966 1 4964 4965 Tricolored squirrel 2 4977 1 4977 American red squirrel 1 4971 Southern flying squirrel 1 4972 4973 Columbian ground squirrel 1 4957 4958 Richardson's ground squirrel 2 4959 4960 1 4954 4959 1 4954 4955 Fat-tailed gerbil 3 Golden spiny mouse 1 Fat sand rat 1 Bushy-tailed jird 1 1 1 Large Egyptian gerbil 1 Greater Egyptian jerboa 1 4975 4976 1 Lesser Egyptian jerboa 1 GenBank accession nos.
(Meriones libycus), jird
carcasses, rodent burrows, and rodent excreta were found at the camel corral.
To attempt to obtain an isolate of the parasite that infected the patient (hereafter referred to as the patient's parasite), whole blood specimens, collected from him in tubes containing the anticoagulant disodium EDTA, were injected into hamsters (Mesocricetus auratus) and jirds. Animal experimentation guidelines were followed.
Attempts to obtain an isolate of the patient's parasite, by injecting specimens of his blood into hamsters and jirds, were unsuccessful (Table 2 footnote).
Attempts to obtain an isolate of the parasite that infected the Austrian patient, by injecting specimens of his blood into jirds, were unsuccessful; the smears of blood from periodic tail snips and PCR analysis of blood obtained by cardiac puncture of the jirds were negative.
The evidence typically has included various combinations of morphologic data, from examination of blood smears; serologic data (usually, but not always, from IFA testing); and data concerning whether jirds or cattle injected with the patient's blood become parasitemic.
burgdorferi s.s.--infected jirds. In each fifth reaction mix, water was added instead of extracted DNA to serve as a negative control.
Although spirochetes readily pass between ticks confined in the same feeding chamber, none do so when the infected ticks feed on the back of a mouse while noninfected ticks are feeding on the mouse's head (3), when attached to different ears of a jird (5), or when the distance separating the infected from the noninfected ticks is 3 cm (4).