The company has developed the capability to design probes to the junction sequence that is formed when two genes fuse to one another.
NanoString president and CEO, Brad Gray, said that with this technology, the new product allows the design of probes for any fusion junction, enabling customers to design and develop full custom sets of probes for their fusion junction sequences of interest.
Because all 10 probes center on the fusion splice junction, in theory, they should give specific signals when fusion transcripts with the correct fusion junction sequence
are present with no substantial hybridization with wild-type partner genes.
We characterized the breakpoint junction sequence
of the [--.sup.THAI] deletion in the original patient described by Winichagoon et al.
Many of these studies looked at successive B-cell lymphomas, using multiplex polymerase chain reaction (PCR) with the BIOMED-2 primer set (10) coupled with capillary electrophoresis to evaluate the pattern of clonal peaks in primary and recurrent tumors to establish clonal relatedness, rather than the exact IGH/IGK V(D)J junction sequences
. This approach, even though widely available for clinical practice, is limited by relying solely on the length of the PCR products to make a determination about whether or not 2 clones are related.
We also estimated the rate of substitution for the C-prM junction sequences
and studied the population dynamics of DEN-3 in the Indian subcontinent.