The primary outcome was the number of keratinocyte
carcinomas overall and, specifically, the number of squamous cell carcinomas (SCCs) before and after the switch.
The muscular layer has an impact on wound closure that in mice is mainly achieved by early contraction, while in humans it relies on keratinocyte
proliferation and migration (re-epithelialization).
To investigate collagen metabolism in keratinocytes
and fibroblasts co-culture under pressure, and study the role of IL-1[alpha] and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.
Human epidermal keratinocyte
cell line HaCaT was obtained from CLS Cell Lines Service (Germany).
Background: Signal transducer and activator of transcription 3 (STAT3) was strongly expressed and activated in psoriatic keratinocytes
(KCs) and correlated with the severity of psoriasis.
Certain drugs, by nonimmunological mechanisms, can cause epithelial damage by dysregulating the activity of enzymes essential for the function of keratinocytes
and for keratinocyte
adhesion to one another and to the basal lamina.
The obtained primary cells were then plated on 6-well tissue culture plates (Costar), precoated with coating matrix (type I collagen, Gibco BRL), cultured using a specific keratinocyte-serum-free media at low [Ca.sup.2+] concentration (<0.07 mM), and supplemented with human keratinocyte
growth factors (Gibco BRL).
The reasons for this are unclear but involve lack of oxygen reaching skin cells, delayed migration of keratinocytes
, decreased collagen synthesis, and also delayed growth of new blood vessels within the wound.
prepared PLGA nanofibre mat with help of polypropylene auxiliary supporter, which makes the scaffold able to maintain long-term integrity without dimensional shrinkage and studied in-vitro on keratinocyte
cells resulted human skin keratinocytes
can proliferate on the scaffold and infiltrate into the scaffold .
E-cadherin at the cell periphery is a determinant of keratinocyte
differentiation in vitro.
Our results suggest that Wnt-CM can enhance the expression of ECM components and cell migration of fibroblasts and thereby accelerate wound closure and hair follicle regeneration, but it inhibits the migratory ability of keratinocytes
. In contrast, [HF.sup.Wnt-CM]-CM (fibroblasts treated with Wnt-CM) can accelerate wound closure and promote keratinocyte
migration; however, the number ofhair follicles did not increase compared to the control group.
The immortalized human skin keratinocyte
cell line, HaCat, from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, was grown in RPMI-1640 medium (Gibco, BRC) supplemented with 10% FBS at 37[degrees]C with 5% C[O.sub.2].