Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction
test, in detection of urogenital Chlamydia trachomatis infection.
Probe amplification Cycling probe technology Ligase chain reaction
The ligase chain reaction
as a primary screening tool for the detection of culture positive tuberculosis.
Barany is best known for developing the ligase chain reaction
(LCR) and ligase detection reaction (LDR) and Universal DNA arrays for detection of genetic diseases and cancer-associated mutations.
Comparison of performance and cost- effectiveness of direct fluorescent-antibody, ligase chain reaction
and PCR assays for verification of chlamydial enzyme immunoassay results for populations with a low to moderate prevalence of Chlamydia trachomatis infection.
Ligase Chain Reaction
(LCR): A technique for the detection of any defined DNA sequence by amplification of ligation products complementary to the target sequence.
The human b-globin gene has been genotyped by several methods, including PCR followed by restriction digestion (1, 2), denaturing gradient gel electrophoresis (3), allele-specific amplification during PCR, and the ligase chain reaction
Analysis of ligase chain reaction
(LCR) products amplified in a silicon chip using entangled solution capillary electrophoresis (ESCE).
The Abbott LCx system for the detection of amplicons after PCR or ligase chain reaction
amplification is in routine use in clinical laboratories for the detection of pathogenic microorganisms like Neisseria gonorrhoeae (24), Chlamydia trachomatis (25), or GB virus C (26).
These methodologies include allele-specific hybridization (27), allele-specific amplification (28), ligase chain reaction
(29), and designed restriction fragment length polymorphism (7).