Nonstandard abbreviations: CpG, cytosine/guanine dinucleotide; M, methylated; U, unmethylated; NGS, next generation sequencing; LCR, ligase chain reaction
; LDR, ligase detection reaction; EpiQ, epiallele quantification without sequencing; HRP, horseradish peroxidase; AML, acute myeloid leukemia; MS-HRM, methylation-sensitive high-resolution melting; MS-HRM, methylation-sensitive high-resolution melting.
The performance of three rapid tests, Testpack Chlamydia (Abbott Laboratories, North Chicago, IL), Surecell Chlamydia (Kodak, Rochester, NY) and Clearview Chlamydia (Unipath, Bedford, UK), was evaluated by comparison to the highly sensitive ligase chain reaction
(LCx Chlamydia, Abbott Laboratories, S.
Probe amplification Cycling probe technology Ligase chain reaction
As with PCR, conceptual errors about ligase chain reaction
(LCR) are also evident (Fig.
Amplicons are amplified products generated by PCR or ligase chain reaction
(LCR) that can contaminate subsequent specimens and cause false-positive results.
The ligase chain reaction
as a primary screening tool for the detection of culture positive tuberculosis.
Becton Dickinson Diagnostic Instrument Systems (Sparks, Md.) is developing strand displacement amplification (SDA), while Abbott Diagnostics (Abbott Park, Ill.) is readying ligase chain reaction
Prospective comparison of the Gen-Probe PACE 2 assay and the Abbott ligase chain reaction
for the direct detection of Chlamydia trachomatis in a low prevalence population.
The human b-globin gene has been genotyped by several methods, including PCR followed by restriction digestion (1, 2), denaturing gradient gel electrophoresis (3), allele-specific amplification during PCR, and the ligase chain reaction
Analysis of ligase chain reaction
(LCR) products amplified in a silicon chip using entangled solution capillary electrophoresis (ESCE).
The Abbott LCx system for the detection of amplicons after PCR or ligase chain reaction
amplification is in routine use in clinical laboratories for the detection of pathogenic microorganisms like Neisseria gonorrhoeae (24), Chlamydia trachomatis (25), or GB virus C (26).