Figure 3(c) shows the high-magnification image of the lipoplex
formed from the metallosurfactant complexation with DNA and Figure 3(d) shows the size of the lipoplex
to be ~75 nm based on the height profile of the AFM cantilever.
To date, numerous nonviral transfection carriers based on cationic lipids and cationic polymers are available on the market (lipoplexes
and polyplexes, resp.).
To our knowledge, no detailed reports have so far been published regarding lipoplex
and polyplex-mediated transfection of DNA into different myeloma cell lines.
Cationic liposomes The presence of HA-DOPE lipid [19-21] conjugate in the liposome composition did not affect the lipoplex
Hashida, "Enhanced DNA vaccine potency by mannosylated lipoplex
after intraperitoneal administration," Journal of Gene Medicine, vol.
Moreover, the encapsulation of CpG ODNs in lipidic particles (i.e., liposomes, lipoplexes
, etc.) results in increased immunostimulatory effects .
Dufay-Wojcicki et al., "Lipoplexes
targeting the CD44 hyaluronic acid receptor for efficient transfection of breast cancer cells," Molecular Pharmaceutics, vol.
This was demonstrated in lung cancer models for delivering miR-29b through cationic lipoplexes
. miR-29b is a tumor suppressor that is downregulated in cancer.
Lee, "MicroRNA delivery by cationic lipoplexes
for lung cancer therapy," Molecular Pharmaceutics, vol.
were then prepared by complexation with a luciferase expressing plasmid (pCMV-luc).
Commercially available cationic lipoplexes
and polyplexes such as the Superfect reagent and lipofection are valued for their limited toxicity, ease of use and ability to transfect a wide range of cell types with relatively higher efficiencies than seen with previously mentioned methods (10), (32).
Miller, "A dialkynoyl analogue of DOPE improves gene transfer of lower-charged, cationic lipoplexes
," Organic and Biomolecular Chemistry, vol.