luciferase


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Related to luciferase: luciferin

luciferase

[lü′sif·ə‚rās]
(biochemistry)
An enzyme that catalyzes the oxidation of luciferin.
References in periodicals archive ?
Luciferase activity was determined 48 h after transfection, using the Dual-Luciferase Reporter Assay System (Promega, USA) following manufacturer's instructions.
The researchers used silica nanoparticles about 10 nanometers in diameter to carry luciferase, and they used slightly larger particles of the polymers PLGA and chitosan to carry luciferin and coenzyme A, respectively.
Luciferin reacted with luciferase, causing it to emit light, while coenzyme A inhibited luciferase activity which promoted the process.
The KeratinoSens[TM] cell line contains a stable insertion of a luciferase gene under the transcriptional control of a constitutive promoter fused with the ARE (Antioxidant Response Element) from the AKR1C2 gene (Lou et al.
Despite its difficulties with absolute quantitation, luciferase has been used to monitor ATP changes in a variety of cells, such as HEK-293 cells, cardiomyocytes, and neurons (Brovko, 2010).
Luciferase SSA reporter assay showed that co-transfection of MSTN TALEN and SSA reporter vector resulted in a 3-fold increase of luciferase activity (p<0.
While mice were still sedated, they were sacrificed by cervical dislocation, livers were dissected and washed in PBS and then homogenized in lysis buffer, and luciferase activity was determined in liver homogenates using the Luciferase Reporter Assay System and a Veritas microplate luminometer.
To assess whether miR-1 directly regulates VEGFA expression by binding the 3'-UTR of VEGFA mRNA in U2OS cells, luciferase reporter vector containing the putative VEGFA 3'-UTR target site for miR-1 and the mutant version were cotransfected with miR-1 mimic or scramble mimic into U2OS cells.
We then showed that Ngn3 could bind to the promoter of Sohlh1andincrease the transcription of Sohlh1 by ChIP and luciferase reporter gene assay.
Anti-GP neutralizing antibodies can inhibit the entry of this pseudoviral particle into cells, thereby inhibiting the expression of luciferase in the assay.