After 24 hrs of incubation, cells were equilibrated at room temperature for 30 min, and then 100 [micro]L of ATP reagent (CellTiter-Glo luminescent cell
viability assay, Promega) was added to each well.
Ara-C toxicity was also assayed with a 3-day cytotoxicity assay using the Cell Titer-Glo[R] Luminescent Cell
Viability Assay kit (Promega, www.promega.
Effect of GSE on cell viability was analyzed by CellTiter Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA) following manufacturer's protocol.
Effect of GSE on viability of fibroblast cells was analyzed by Cell titer Glo Luminescent Cell Viability Assay (Promega, USA).
liquid handling workstation has been used to automate Promega Corp.'s CellTiter-Glo luminescent cell
Among its varied applications is its use in luminescent cell
viability and quantitation, cell proliferation and cytotoxicity, and luminescent reporter gene assays.
Existing 2-D, or single-view, in vivo luminescence imaging systems are limited in terms of spatial resolution, and the ability to quantitatively estimate the number of luminescent cells
within the subject.
Contag and his coworkers at the Stanford University School of Medicine have now performed a similar feat using luminescent cells
of human cancers.
Without luminescent cells
only one strike took place, and this at greater than 29 min.