008 g glucose, 1 g BSA) and then exposed for 30 min to 100 [micro]g/mL of cyanobacterial lysate
and a 1:1,000 dilution of high-affinity IgE receptor (FceR1) in Tyrod's buffer.
Flowcytometric analysis of apoptosis: At 24, 48 and 72 h post inoculation with culture supernatant and cell lysate
, HeLa cells on 60-mm tissue culture plate were treated with 1 x trypsin-EDTA (Invitrogen, USA) for 10 min at 37[degrees]C; cells were then removed from plate by gentle scrapping, dispensed into cold PBS and washed twice.
Among the 50 frozen vaginal/anal lysates
, 25 were identified as positive and 25 identified as negative for GBS by the RT-PCR with the BD GeneOhm Strep B assay kit.
After visiting the official website of Creative Biomart, the reporter found that it has thousands millions of over-expression lysates
The placebo-controlled study aimed to evaluate the safety and efficacy of Cutaneous Lysate
in the treatment of provoked vestibulodynia; a form of a vulvar pain symptom called vulvodynia.
The cell lysates
(100 [micro]g/ 100 [micro]l protein) were incubated with specific substrate Ac-DEVD-AMC for caspase-3, IETD-pNA for caspase-8, or LEHD-pNA for caspase-9 for 1-2 h at 37[degrees]C.
Alex Vodenlich, Gentel's President and CEO, said of the product, "This launch expands the applications of APiX technology to include not only antibody and protein arrays on clear glass slides, but also tumor lysate
arrays on porous nitrocellulose surfaces.
NADPH oxidase activity was determined in PMA-stimulated neutrophil lysate
(Jones and Hancock, 1994).
microarrays (also called reverse phase protein microarrays): Complex samples such as lysates
from tissues or cells are arrayed onto the solid phase, and then probed with antibodies to a target protein of interest.
com)-- After expansion, Creative BIolabs provides immunoassay kits for quantitative detection of enzymes, antibodies, antigens, receptors, inhibitors, peptides and small molecules from cell culture supernatant, tissue homogenate, cell lysate
, serum, plasma, ascites, cerebrospinal fluid and urine samples that are collected from either preclinical animal studies or clinical human studies.
Before assay, the lysate
was centrifuged at 20 000gfor 30 min at 4[degrees]C, the supernatants were collected, and the telomerase activity estimated by the TRAP assay described below.
After seven days of (131)I treatment, RBC lysate
was prepared and its antioxidant status assessed.