Before use, the AB-8 macroporous resin
was soaked in 95% ethanol for 24 h and washed several times using the deionized water.
(2012), the yield was 84.3% in NKA macroporous resin
. For Palomino-Romero et al.
Their total and individual content of flavonoids were determined by combining macroporous resin
chromatography with high-performance liquid chromatography (MPRC-HPLC) method, which was developed for the simultaneous enrichment and analysis of flavonoids from lotus leaves, recently .
The ferments were centrifuged (10,000g, 30min, 4[degrees]C), and the cell-free supernatant was absorbed with the macroporous resin
D4020 (average pore diameter 100-105 [Angstrom], specific surface area of 540-580 [m.sup.2]/g, Nankai University Chemical Factory, China).
The highly polar antioxidants were firstly isolated and purified from Chirita longgangensis by combination of macroporous resin
and HSCCC (Duan et al., 2014).
Methods: Chemical constituents were extracted and purified by ethanol reflux extraction and chromatographic separation technology including D-101 Macroporous resin
, silica gel, Sephadex LH-20 and preparative thin-layer chromatography.
TLC silica gel GF-254 and column chromatography silica gel (200-mesh) were both manufactured by Xindi Chemical Plant in Yantai; RPTLC plates were products of Merck, Germany; Sephadex LH-20 was a product of Pharmacia; RP-18 reversed phase silica gel was a product of Great Door, Germany; MCI GEL CHP-20P was a product of Mitsubishi Chemical; and D101 macroporous resin
is a product of Dashi Technology Development Co., Ltd.
Crude extract of summer green tea obtained by hot water extraction was passed through columns of polyamide and NKA-II macroporous resin
respectively, resulting in tea polyphenols-enriched fraction (96.27 A+- 1.78%), theanine-enriched fraction (99.02 A+- 0.43%) and caffeine-enriched fraction (99.25 A+- 0.36%).
The macroporous resins
NKA-11 was used for chromatography of the crude extract L The macroporous resin
NKA-11 is a kind of cross-linked polystyrene with polarity.
giganteum could significantly prolong the survival time of myocardial hypoxia mice, fractions eluted by 50% EtOH in NKA-9 type macroporous resin
could significantly prolong the survival time in myocardial hypoxia mice, but also to improve the biochemical indicators of myocardial ischemia mice, so extraction process using 60% EtOH should be a more reasonable extraction method, the eluted material with 50% EtOH in macroporous resin
should be the active friction.
The extract was concentrated under reduced pressure, and then dissolved in 30% ethanol to give 1 g/ml (crude medicine/volume) sample liquor, and then this liquor was passed through AB-8 macroporous resin
column with 1 time the volume which was comparable to the weight of macroporous resin
Actually, fast kinetics can be expected in applications of macroporous resins
. In addition, high ligand concentration, 1 mmol [g.sup.-1] resin, used in impregnation may increase selectivity of the resin which can also be a reason for fast sorption rate.