How IVM works: The immature bovine oocytes are released out from their follicles and are cultivated in regular maturation medium, following that oocytes attain the first meiotic division stage (Sirard and Coenen, 2006).
Oocyte maturation: For fruitful fertilization and embryo growth, the oocyte maturation is an essential event.
In vitro maturation or in vitro fertilization for women with polycystic ovaries?
Development of in vitro maturation techniques for clinical applications.
The COCs were then washed three times in 100 ul of maturation
medium TCM-199 (Earle's salts) with 10% FCS, 0.5 mM Na-Pyruvate, 0.02 IU/ml follicle-stimulating hormone (FSH), 0.023 IU/ml luteinizing hormone (LH), 1 ug/ml E2, 100 uM cystamine, 50 ug/ml gentamycin and 0% SFF (Control: Group A), 10% SFF (Group B), 20% SFF (Group C) or 40% SFF(Group D).
Machaca, associate dean of research at WCM-Q, who has led the research, said: "The discovery that the VLDL Receptor plays an essential role in oocyte maturation
was quite unexpected and reveals a novel function for this class of proteins that was previously not appreciated.
They related these stress levels to the maturation
of the prefrontal cortex, amygdala and hippocampus.
After classification, COCs were washed three times in Hepes-buffered TCM-199 and then twice in maturation
After this process, GV oocytes were placed into in vitro maturation
media and MII in culture media, respectively.
However, the success of IVM of COCs in coculture also depends on the choice of a suitable medium to allow oocyte maturation
and the survival and potency of the hWJ-MSCs.
In vitro maturation
of oocytes has been suggested as an alternative approach to conventional IVF as it completely avoids the risk of OHSS .
After recording the Metaphase II (MII) oocyte maturation
rate, the oocytes were fertilized in vitro.