HCl, petroleum ether, CaCO3 were purchased from Fluka Chemicals and chloroform, tris, KCl, mercaptoethanol
were purchased from Merck.
These vitelline envelope proteins could be extracted by acetic acid, which yielded a higher amount of proteins than other chemical treatments, such as mercaptoethanol
There is no indication that the 6M urea and mercaptoethanol
used as wash solutions were removed by dialysis.
He used Mercaptoethanol
capping agent to prevent nanoparticles from being lumped through the process.
In some cases mercaptoethanol
, a powerful disrupter of disulfide bonds, was added to the urea solution, or to a phosphate buffer, as a final, often long-term, leach.
After rinsing and separation, sulphur bonds were created on the surface of the nanoparticles by using mercaptoethanol
Preantral follicles were dissected out under stereo zoom microscope and washed thrice in preservation medium consisting of TCM-199 (90 %) supplemented with follicular fluid (10 %), Mercaptoethanol
(10 %) and gentamicin (50 [micro]g/ml oocytectomized follicles and were washed twice in culture medium before they were use).
II], including sodium sulfite, sodium hydrogen sulfite, inorganic salts comprising a metal ion, hydrazine hydrate, mercaptoethanol
, tetrahydrofuran, dihydroanthracene, 2,3-dimethylbutadiene, silane compounds, borane compounds, aldehydes, and derivatives of such compounds.
The inhibition by DTNB was released by adding 10 mmol/L mercaptoethanol
, and the sample incubated for 30 minutes.
A similar work by Yusof et al  did purify the patatin-like-protein (exhibits lipid acyl hydrolase activity) from C-serum of fresh latex by using 50 mM Tris-HCl buffer, pH 8 plus 5 mM 2- mercaptoethanol
as the protein buffer.
2 to 2% [beta]- Mercaptoethanol
before use v/v); Polyvinyl pyrrolidone (MW 40000); Washing Solution I (0.
5 per cent each of SDS and mercaptoethanol
was kept in a boiling water bath for 3 min in a tightly stoppered vial.