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The perfect results about virus eradiation from the propagative material are obtained by the combination of thermotherapyin combination with meristem culture; where alone meristem culture fails to produce successful results (Waswa et al., 2017).
The classification system used was based on vegetative branching meristem location.
RGFR1 and RGFR2 are predominantly expressed in the proximal meristem including the elongation zone and gradually decrease in expression towards the differentiation zone.
The meristems were extracted under aseptic conditions and incubated in MS medium, supplemented with sucrose at 30 g [L.sup.-1] and the phytagel solidifier at 2.5 g [L.sup.-1], pH adjusted to 5.8 [+ or -] 1 before autoclaving (sterilization) at 120 [degrees] C with 1 Kgf [cm.sup.-2] for twenty minutes.
Thus, the bifunctional YABBY transcription factors have an important role in driving the evolution of the leaf and gynoecium, as well as in the initiation, growth, and structural organization of almost all aboveground lateral organs, and in the control of shoot apical meristem organization and activity.
Among the preservatives stand out potassium benzoate, sodium benzoate, potassium nitrate, cytotoxic and genotoxic effects to normal cells of human peripheral blood (Mpountoukas et al., 2010, Zequin, Yuzbagioglu, Unal, Yilmaz, & Aksoy, 2011), boric acid, citric acid, potassium citrate and sodium citrate, cytotoxic and genotoxic to root meristem cells of A.
Direct systems of regeneration through the culture of organized meristems normally produce true-to-type plants.
To induce the meristems multiplication, the isolated meristem and the meristem with adjacent tissue were inoculated on culture MS medium (MURASHIGE; SKOOG, 1962), supplemented with 20 g [L.sup.-1] sucrose, 1 mg [L.sup.-1] indoleacetic acid (IAA) and 0.32 mg [L.sup.-1] benzylaminopurine (BA).
In light of the fact that China owes in part, its sweet potato success story to the meristem culture technique [11], the objective of the study was to develop a regeneration protocol for Zimbabwean sweet potato (cv Brondal) using meristems as explants.
[17] showed that the method of staining with tetrazolium can be used to determine the most damaged parts of the germ, the results show that the root meristem is more damaged compared to the meristem for soy elderly seeds and barley.
Finally, an apical, conspicuous multicellular meristem was developed (Fig.